藏羚羊mtDNA D-loop区遗传多样性研究  

Study on Mitochondrial DNA Genetic Diversity of Tibetan Antelope

作　　者：周慧[1] 李迪强[2] 张于光[2] 易湘蓉[1] 刘毅[1] 

机构地区：[1]湖南农业大学动物科学与技术学院,湖南长沙410128 [2]中国林业科学研究院森林生态环境与保护研究所,北京100091

出　　处：《遗传》2006年第3期299-305,共7页Hereditas（Beijing)

基　　金：国家科技部社会公益项目"濒危动物保护技术"(编号:2001DIB100058);国家"十五"科技攻关项目(编号:2001BA510B10)资助~~

摘　　要：该研究采用非损伤性DNA基因分型技术，对可可西里地区10个藏羚羊（Pantholops hodgsonii）个体的mtDNA非编码区部分片段（444～446bp）进行了序列分析，结果显示A、T％含量（61．8％）明显高于G、C％含量（38．2％），共发现10种单倍型，包括48个多态位点，其中转换位点44个、颠换位点1个、插入位点1个、缺失位点2个。单倍型间平均遗传距离为0．031，单倍型多态性（h）为1．000，核苷酸多态性（π）为2．96％。说明藏羚羊线粒体控制区存在着丰富变异，最后从藏羚羊的生态习性及地理分布两方面对这一结果进行了分析探讨。With noninvasive DNA genotyping technology, analyzing mitochondrial DNA variation, those samples are we investigated genetic diversity of Tibetan antelope by collected from Hoh Xil National Nature Reserve, Qinghai province, China. A total of 444-446 bp of the mitochondrial control region was sequenced form 10 individuals. The results showed that A% ＋ T% （61.8 % ） was higher than C% ＋ G% （38.2 % ） obviously. Ten haplotypes were identified in the 10 samples. The result showed 48 polymorphic sites after comparisons of the 10 haplotype samples, among them, including 44 transitions, 1 transversion, 1 insertion and 2 deletions. The average genetic distance of haplotypes is 0. 031. The haplotypic diversity is 1. 000. The nucleotide diversity is 0. 0296. The average ru-nber of nucleotide differences is 13. 127. It showed that the Tibetan antelope population has high variation in mitochondrial D-loop sequence.

关 键 词：藏羚羊 线粒体D-LOOP区 单倍型 遗传多样性 

分 类 号：Q953[生物学—动物学]