三七皂苷Rg1对脑缺血后大脑皮质和海马BDNF蛋白的影响  

Role of gisenoside-Rg1 on BDNF protein incerebrum cortex and hippocampus after brain ischemia

作　　者：詹合琴[1] 尹志奎[1] 翟成凯[2] 吴蓝鸥[3] 

机构地区：[1]新乡医学院,河南新乡453003 [2]新乡市第一人民医院 [3]昆明医学院

出　　处：《山东医药》2006年第5期9-10,共2页Shandong Medical Journal

基　　金：河南省教育厅自然科学基金项目(2006310013)Δ通讯作者

摘　　要：目的探讨三七皂苷Rg1对脑缺血后皮质和海马脑源性神经营养因子（BDNF）蛋白的影响。方法取60只成年雄性SD大鼠建立大脑中动脉缺血模型，2h后进行缺血再灌注并随机分为1、2、3、4、5组各12只，分别给予生理盐水5mg／kg、尼莫地平15mg／kg及三七皂苷Rg150、100、200mg／kg腹腔注射，于第1、3天用4％多聚甲醛饱和苦味酸进行心脏灌注并处死动物，制作脑组织冰冻切片，采用兔抗BDNF（1：500）抗体进行免疫组化ABC法染色，观察各组不同时间大脑皮质和海马BDNF蛋白灰度值及阳性神经元数量。结果大鼠大脑皮质和海马均可见BDNF蛋白阳性表达；3～5组BDNF蛋白的灰度值低于1、2组（P〈0．05、〈0．01），阳性神经元数量显著高于1、2组（P〈0．05、〈0．01）；3、4组大脑皮质BDNF灰度值和阳性神经元数目均低于海马（P〈0．05、〈0．01）。结论三七皂苷Rg1能增加大脑皮质和海马BDNF阳性神经元的数量和蛋白含量，两部位的观察指标有显著性差异。其机制可能是BDNF不仅存在于阳性神经元中，亦可能是不同部位脑组织对药物和脑缺血的反应性不同。Objective： To explore the role of Gisenoside-Rg1 on BDNF protein in cerebrum cortex and hippocampus after brain ischemia. Methods. The cerebral infarction in the territory of middle cerebral artery models were established by using 60 adult male SD rats, 2 hours later, these rats were performed reperfused and divided randomly into five groups, each with 12 rats. Five groups were performed intraperitoheal injection with saline （5mg/kg）, nimodipine （15mg/kg）, Gisenoside （150mg/kg） Gisenoside （100mg/kg） and Gisenoside （200mg/kg） respectively. Constitution were prepared with frozen section and the sections were stained by immunohistochemistry ABC method by using BDNF （1 ： 500） antibody. The BDNF protein grayworth and positive neurons amounts were observed. Results： BDNF protein was observed both in cerbrum cortex and hippocampus. The BDNF grayworth in groups 3-5 is lower than that in group 1 and group 2 （P〈0. 05,〈 0. 01）. But the positive neurons amounts was significanfly higher than that in the latter. The BDNF grayworth arid the positive neurons amounts in cerebrum cortex were both Tower than that in hippocampus in group 3 and group 4 （P〈 0. 05, 〈0. 01）. Conclusion： Gisenoside-Rg1 can increase the protein in BDNF-positive neurons both in cerebrum cortex and hippocampus. There is a sigmfficant difference between the cerbrum cortex and hippocumpus. The possible mechanism is not only that BDNF exists in positive neurons but also different parts of the brain have different reaction to drugs and cerebral ischemia.

关 键 词：三七皂苷RG1 脑缺血 大脑皮质 海马 

分 类 号：R743.9[医药卫生—神经病学与精神病学]