发酵时间和乙二酸前体对小核盘菌生物除草剂致病力的影响  

Effects of fermentation duration and precursors of oxalic acid on the virulence of herbicide of Sclerotinia minor

作　　者：许修宏[1] AKWatson MPTeshler 

机构地区：[1]黑龙江省农业科学院博士后工作站 [2]加拿大麦吉尔大学植物科学系

出　　处：《植物保护学报》2005年第4期421-424,共4页Journal of Plant Protection

基　　金：黑龙江省科技计划国际科技合作项目(WC03210);黑龙江省自然科学基金项目(C2004-20);黑龙江省教育厅海外学人项目(1055HQ033)

摘　　要：以粉碎的大麦颗粒为培养基,采用固体发酵法制作小核盘菌 Sclerotinia minor Jagger 生物除草剂,研究发酵时间和乙二酸生物合成前体物质对除草菌的生活力、致病力及乙二酸产量的影响,以期为提高除草菌除草效率提供理论依据。发酵7天的液体菌种生产的除草菌生活力达到最大,为65.0 mm,发酵6天的液体菌种生产的除草茵致病力达到最大,为55.8 mm;固体发酵6天的除草菌的生活力、致病力和乙二酸产量均达到最大,分别为64.3 mm、54.3 mm 和0.131 mg/g;添加乙二酸前体对除草菌生活力没有明显影响。添加浓度为56mmol/L 的琥珀酸钠和柠檬酸后,除草菌中乙二酸产量分别比对照增加了64.4％和39.7％,除草菌的致病力分别增加了12.4％和5.4％,乙二酸产量与致病力之间存在正相关性。Barley grits were used to produce herbicidal Sclerotinia minor Jagger by solid fermentation. Effects of fermentation duration and precursors of oxalic acid on viability, virulence and oxalic acid production of S. minor were measured to provide data for improving its herbicidal effect. S. minor had the highest viability of 65.0 mm when mycelium from liquid fermentation for 7 days was used and the highest virulence of 55.8 mm when mycelium from liquid fermentation for 6 days was used. Herbicide produced from 6 days solid fermentation had the highest viability, virulence and oxalic acid production, which were 64.3 mm, 54.3 mm and 0.131 mg/g respectively. Precursors of oxalic acid had no significant influence on the viability of S. minor. Addition of 56 mmol/L sodium succinate and citric acid raised the oxalic acid production in S. minor by 64.4% and 39.7% respectively and raised its virulence by 12.4% and 5.4% respectively. Positive correspondence was found between oxalic acid production and virulence.

关 键 词：发酵时间 乙二酸前体 小核盘菌 生物除草剂 致病力 

分 类 号：S482.4[农业科学—农药学] TS252.54[农业科学—植物保护]