P120ctn在MDS大鼠骨髓中的表达及其与血管新生的关系  

Expression of P120ctn in a Rat Model for Myelodysplastic Syndrome and Its Correlation with Angiogenesis

作　　者：罗小华[1] 刘文励[1] 吴颖[1] 罗莉[1] 孙汉英[1] 周剑锋[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,湖北武汉430030

出　　处：《中国血液流变学杂志》2005年第4期536-539,共4页Chinese Journal of Hemorheology

摘　　要：目的探讨P120ctn(P120连环素)在MDS(骨髓增生异常综合征)大鼠骨髓中的表达及其与血管新生的关系。方法在用DMBA诱导法建成MDS大鼠模型后,用免疫组织化学方法(SP)检测骨髓P120ctn的表达,同时用因子Ⅷ相关抗原标记骨髓组织微血管。用Image-ProPlus图像分析仪对P120ctn的表达做定量分析及测量微血管密度,并对两者作相关性分析。结果P120ctn主要表达于骨髓基质细胞和微血管内皮细胞,MDS组骨髓P120ctn的表达及微血管密度(MVD)明显高于正常对照组大鼠,差异均有显著意义(P<0.01或P<0.05),并且两者存在明显正相关(Pearson相关系数r=0.652,P=0.023)。结论MDS大鼠骨髓存在明显的血管新生,P120ctn的表达呈现明显升高,提示P120ctn的表达与血管新生存在某种关系。Objective To investigate the expression of P120 catenin in the bone marrow of a rat model for myelodysplastic syndrome and its correlation with angiogenesis of the model.Methods After establishment of the model induced by the chemical mutagen dimethylbenzantharcene（DMBA）,The expression of P120ctn in bone marrow sections was measured immunohistochemically,and the bone marrow microvessels were highlighted using anti-F Ⅷ-related antigen staining.Both were quantified by Image-Pro PlusL-and were analyzed their correlation statistically.Results P120ctn can be detected in stromal cells and microvessel endothelial cells of bone marrow. A sisnificant increase inangiogenesis and the expression of P120ctn were seen in MDS group compared to controls （P〈0.01 or P〈0.05）. At the same time,the expression of P120ctn was positively related to angiogenesis in bone marrow.Conclusion The expression of P120ctn and angiogenesis were up-regulated in the model for myelodysplastic syndrome and both changes were correlated to some extent.

关 键 词：P120连环素 骨髓增生异常综合征 血管新生 微血管密度 

分 类 号：R733.3[医药卫生—肿瘤]