多重耐药铜绿假单胞菌氨基糖苷类修饰酶基因及耐消毒剂基因的检测  

Detection of AMEs Gene and Disinfect-resistance Genes in Multi.Drug resistant Pseudomonas Aeruginosa

作　　者：周丽珍[1] 宫玲玲[1] 王继东[1] 

机构地区：[1]江南大学附属无锡市第五人民医院,江苏无锡214073

出　　处：《世界感染杂志》2005年第6期475-477,共3页World Journal of Infection

摘　　要：目的了解我院多重耐药铜绿假单胞菌中氨基糖苷类修饰酶编码基因及耐消毒剂基因的存在状况。方法聚合酶链反应（PCR）法对多重耐药的铜绿假单胞菌进行氨基糖苷类修饰酶（AMEs）基因、qacE△1-sul1耐消毒剂基因的检测。结果本组7株多重耐药的Pa菌分别检出aac（6’）-Ⅰ、aac（6’）-Ⅱ、ant（3″）-Ⅰ和ant（2″）-Ⅰ等4种氨基糖苷类修饰酶基因和qacE△1-sul1型耐消毒剂基因。结论多重耐药铜绿假单胞菌存在多重耐药基因。Objective To comprehend the situation of aminoglycosides modifying enzyme encoding genes and disinfect-resistance genes in multi-resistant drug pseudomonas aeruginosa isolated from our hospital. Methods The aminoglycosides modifying enzymes （AMEs） gene and disinfect-resistance genes were determined by poiymerase chain reaction （PCR）. Results aac（6＇）-Ⅰ、aac（6＇）-Ⅱ、ant（3″）-Ⅰ and ant （2＂）- Ⅰ of AMEs genes and qacE△1-sul1 of disinfect-resistance genes were detected in seven strains of multi-resistant drug pseudomonas aeruginosa. Conclusion There are multi-resistant drug genes in multiresistant drug pseudomonas aeruginosa.

关 键 词：铜绿假单胞菌 多重耐药 基因检测 

分 类 号：R378.991[医药卫生—病原生物学]