不同年份对虾白斑综合征病毒基因组差异的微阵列分析  

Microarray analysis of genomic variations of white spot syndrome virus in different years

作　　者：吴常嵩[1] 杨丰[2] 

机构地区：[1]厦门大学生命科学院,厦门361005 [2]国家海洋局第三海洋研究所,厦门361005

出　　处：《高技术通讯》2006年第2期201-203,共3页Chinese High Technology Letters

基　　金：国家863计划（2001AA626020）及国家自然科学基金（40276038）资助项目.

摘　　要：根据对虾白斑综合征病毒（white spot syndrome vires，wssv）全基因组序列的分析与应用，设计了190对引物，覆盖全基因组绝大多数可预测的开放读框（0RF）。扩增相应基因片段，在尼龙膜上点样，制备成为DNA微阵列。收集了1996年和2002年感染阳性对虾，分别提取纯病毒基因组DNA，用地高辛标记后与DNA微阵列杂交。与1996年病毒株相比较，2002年病毒株缺失了wsv479、wsv482、wsv489和wsv493。运用PCR技术验证了DNA微阵列杂交结果的可靠性。Based on the analysis and availability of the entire sequence of white spot syndrome virus （WSSV） genome, 190 primer pairs were designed for amplifying of its most potential ORFs. The PCR amplified products were spotted onto the nylon membrane to generate arrays. WSSV genomic DNA was isolated respectively from two WSSV isolates prepared in year 1996 and 2002 and labeled with DIG-11-dUTP. Each labeled probe was hybridized to a microarray. Compared to the WSSV-1996 isolate, the WSSV-2002 isolate has the deletion in wsv479, wsv482, wsv489 and wsv493. Polymerase chain reaction （PCR） was performed to validate the microarray results.

关 键 词：日本对虾 螯虾 白斑综合征病毒 DNA微阵列 PCR 

分 类 号：S945.1[农业科学—水产养殖] S511[农业科学—水产科学]