多粘芽孢杆菌JW-725抗菌活性物质及其发酵条件的初步研究  

Fermentation of Bacillus polymyxa JW-725 and preliminary extraction of the antifungal substances

作　　者：赵德立[1] 曾林子[1] 李晖 陈金瑞 刘成君[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]西藏高原生物研究所,拉萨850001

出　　处：《植物保护》2006年第1期47-50,共4页Plant Protection

基　　金：四川省科技厅应用基金项目(03JY029-067-2)

摘　　要：多粘芽孢杆菌JW-725对柑橘青霉具有很强的拮抗作用。通过5因素4水平正交试验明确,应用LB培养基培养,初始pH 9.0,30℃,通气量100 mL,接种量为10%,140 r/min振荡培养72 h时的发酵液具有最佳抗菌活性。该菌胞外抗菌物质可用乙酸乙酯单一溶剂提取。Bacillus polyrnyxa JW-725 has a significant antifungal effect on Penicillium italicum. The flve-factor and 4-grade orthogonal experiments found that the fermentative liquid had the strongest antifungal effect, with the following fermentative eonditions：LB media with initial pH9. 0, 30℃, 72 h, 10% inoculum and 100 mL culture in a 500 mL bottle, Further studies showed that the strain secreted an extracellular antifungal product that could be extracted preliminarily by ethyl acetate.

关 键 词：生物工程 多粘芽孢杆菌 抗真菌活性物质 发酵条件 

分 类 号：S482.212[农业科学—农药学]