小鼠脾脏对肝癌细胞作用的实验研究  

EXPERIMENTAL STUDY OF SPLEEN ACTING ON MOUSE′S LIVER CARCINOMA CELLS

作　　者：李斌[1] 张培建[1] 陶立德[1] 王跃全[1] 王红鲜[1] 

机构地区：[1]江苏省扬州市第一人民医院普通外科研究室,江苏扬州225001

出　　处：《实用临床医药杂志》2006年第1期39-40,共2页Journal of Clinical Medicine in Practice

摘　　要：目的研究脾脏对肝癌细胞的抑制作用。方法小鼠用二乙基亚硝胺(DENA)灌胃诱发肝癌,同时分组给予静脉注射氯化钆(GC)、切脾等不同处理,制作动物模型。试验第12周处死模型小鼠并取材,对标本进行病理学检查和腹腔内巨噬细胞的活性测定。结果A组、B组和C组腹腔内巨噬细胞活性检测、小鼠肝表面最大癌结节平均直径均有显著差异(P<0.05)。结论不仅肝脏本身的Kupffer细胞对肝细胞的癌变过程具有抑制作用,而且脾脏也起着不可忽视的影响,在氯化钆阻断枯否细胞(Kupffer细胞)的活性的前提下,脾脏对肝癌细胞有明显的抑制作用。Objective To explore the influence of spleen toward liver carcinoma cells. Methods Liver carcinoma mouse models were induced by pouring DENA, injecting GC and cutting spleen. The test applied both pathological scope of mouse＇s liver turnout and determination of acid phosphatase of phagocytic cells to evaluate effects of spleen toward liver carcinoma. Results Both M activity and carcinoma code diameter had a significant difference between group C and group B. Conclusion Spleen had a constrained effects on liver carcinoma cells, based on GC restricted activity of Kupffer cells.

关 键 词：脾脏 氯化钆 巨噬细胞 枯否细胞(Kupffer细胞) 

分 类 号：R735.7[医药卫生—肿瘤]