在BLMA_5-Fe^(2+)体系中肌肽对DNA氧化损伤的保护作用  

PROTECTION FUNCTIONS OF CARNOSION ON DNA OXIDANT DAMAGE INDUCED BY BLMA_5-Fe^(2+) SYSTEM

作　　者：黄进[1] 杨国宇[1] 李宏基[1] 王艳玲[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002

出　　处：《河南工业大学学报（自然科学版）》2006年第1期18-20,共3页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南省杰出青年科学基金资助项目(0312001500);教育部科学技术研究重点项目(204086)

摘　　要：以BLMA5Fe2+体系切割双链DNA产生MDA物质为基础,采用TBA法检测了肌肽对DNA氧化损伤的保护作用,同时以甘露醇、苯甲酸钠为对照,并对肌肽的抗氧化机理做了初步探讨.结果发现肌肽对DNA损伤的保护作用明显优于甘露醇和苯甲酸钠.其抗氧化曲线与L-组氨酸相似,因此推测其抗氧化性与其所含的咪唑环密切相关.DNA can be cleaved by BLMA5-Fe^2 ＋ system to produce MDA, this DNA damage can be observed by TBA reaction. The antioxidant ＂abilities of carnosine, L-histidine, β-alanine, Mannitol and Benzoate were studied with TBA method. The protecting mechanism of carnosine in DNA oxidant damage was analyzed. The results suggested that carnosine has a better protective effect than Mannitol and Benzoate in the right system. Its antioxidant curve is similar to L-histidine, which implied that the antioxidant ability of carnosine is closely bound up with its imidazole cycle.

关 键 词：肌肽 DNA BLMA5-Fe^2+体系 氧化损伤 

分 类 号：TS201.2[轻工技术与工程—食品科学]