槲皮素及异鼠李素对去甲肾上腺素促人血管平滑肌细胞增殖的抑制作用  

The Inhibition of Quercetin and Isorhamnetin on Norepinephrine Stimulated Proliferation of Cultured Human Vascular Smooth Muscle Cell

作　　者：陈维[1] 章茂顺[2] 王家良[2] 符宗胤[2] 朱福[3] 

机构地区：[1]上海交通大学医学院附属第三人民医院心内科,上海市201900 [2]四川大学华西医院,四川省成都市510041 [3]上海市南汇区中心医院心内科,上海市201300

出　　处：《中国动脉硬化杂志》2005年第6期749-752,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的观察槲皮素、异鼠李素和去甲肾上腺素对培养人血管平滑肌细胞增殖的影响.方法利用培养的人主动脉平滑肌细胞,采用细胞计数和3H-胸腺嘧啶掺入法,观察槲皮素、异鼠李素和去甲肾上腺素对血管平滑肌细胞增殖、DNA合成的影响,以及槲皮素、异鼠李素和酚妥拉明对去甲肾上腺素促血管平滑肌细胞增殖、DNA合成的影响.结果槲皮素有明显抑制血管平滑肌细胞增殖和DNA合成的作用,而异鼠李素作用较弱.在所观察1～200 μmol/L的浓度范围内,槲皮素和异鼠李素均在200 μmol/L浓度发挥了最大的抑制作用,其抑制作用有剂量依赖关系.去甲肾上腺素有促进血管平滑肌细胞增殖、DNA合成的作用, 而这些促进作用能被酚妥拉明所阻滞.槲皮素和异鼠李素均呈剂量依赖性抑制去甲肾上腺素促血管平滑肌细胞增殖和DNA合成的作用, 且槲皮素和异鼠李素有明显的协同作用.槲皮素和异鼠李素对去甲肾上腺素刺激作用的抑制明显强于酚妥拉明. 槲皮素和异鼠李素对血管平滑肌细胞无细胞毒性作用.结论槲皮素和异鼠李素是细胞毒性低的天然黄酮类物质,对血管平滑肌细胞增殖、DNA合成尤其是对去甲肾上腺素刺激的血管平滑肌细胞增殖、DNA合成有较强的抑制作用.Aim To observe the role of quercetin （QUE）, isorhamnetin （ISOR） and norepinephrine （NE） on the proliferation of human aortic vascular smooth musde cell （VSMC）. Methods The effects of QUE, ISOR and NE on these VSMC growth and DNA synthesis was investigated by cell counting, ^3H-thymidine incorporation. Results QUE could inhibit proliferation and DNA synthesis of VSMC but these effects of ISOR was weaker than that of QUE. When VSMC were treated by 1 - 200 μmol/L of QUE or ISOR, the best inhibitory effects occured at 200 μmol/L. There were dose-dependent inhibitory effects in different concentration of QUE and ISOR. NE 10μmol/L could stimulate proliferation and DNA synthesis of VSMC. The effects could be inhibited by phentolamine（Ph）. QUE and ISOR markedly inhibited proliferation and DNA synthesis of VSMC induced by NE in a dose dependent manner. At the same time QLrE and ISOR had cooperative effects on inhibition d the stimulation of NE. QUE and ISOR also had more powerful inhibitory effect on proliferation and DNA synthesis of VSMC co-incubation with NE than Ph. There were no cyto-toxic effects on VSMC treated with QUE and ISOR. Conclusion These results indicated that QUE and ISOR had no tyro-toxic effect on VSMC cultured in vitro and could effectively inhibit the proliferation and DNA synthesis of VSMC, especially inhibit the proliferation and DNA synthesis of VSMC stimulated by NE.

关 键 词：病理学与病理生理学 抑制血管平滑肌细胞增殖 ^3H-胸腺嘧啶掺入法 槲皮素 异鼠李素 去甲肾上腺素 细胞计数法 

分 类 号：R363[医药卫生—病理学]