核糖体蛋白L5在胃癌中的表达及功能  

Expression and functional study of ribosomal protein L5 in gastric cancer

作　　者：刘骥[1] 李纪鹏[1] 陈冬利[1] 张洪伟[1] 王为忠[1] 

机构地区：[1]中国人民解放军第四军医大学西京医院胃肠外科,陕西省西安市710032

出　　处：《世界华人消化杂志》2005年第23期2731-2735,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨核糖体蛋白L5(ribosomal protein L5,RPL5) 在胃癌细胞中的表达及对胃癌细胞生长的影响．方法:Western blot检测RPL5在胃癌细胞系中的表达, 构建RPL5特异性siRNA载体,转染细胞,Western blot进行鉴定,MTT方法和流式细胞术检测转染细胞的生长变化．结果:RPL5在胃癌细胞系AGS、MKN45、SGC7901、 MGC803中的表达均明显强于在GES-1和正常胃黏膜上皮中的表达．成功构建RPL5特异siRNA载体U6- RPL5A和U6-RPL5B,转染AGS细胞,进行稳定筛选,发现U6-RPL5A能显著抑制RPL5的表达,其相应的细胞系AGS-U6-RPL5A的生长速度减慢．细胞周期检测结果显示AGS-U6-RPL5A细胞中处于增殖期的细胞减少了约5％．结论:对RPL5功能的进一步深入研究可能会有助于胃癌的诊断和治疗．AIM： To investigate the expression of ribosomal protein L15 （RPL5） in gastric cancer as well as its effect on the proliferation of gastric cancer cells. METHODS： The expression of RPL5 was detected in gastric cancer cell lines AGS, MKN45, SGC7901 and MGC803 by Western blot. The specific siRNA vector of RPL5 was constructed and then transfected into AGS cells. The expression of RPL5 in the transfectants was examined by Western blot. The growth of transfected cells was evaluated by MTT assay and flow cytometry. RESULTS： The expression of RPL5 in gastric cancer cell lines AGS, MKN45, SGC7901 and MGC803 were all significantly higher than that in GES-1 and normal epithelial cells of gastric mucosa. The specific siRNA vectors of RPL5, named U6-RPL5A and U6-RPL5B, were successfully constructed and transfected into AGS cells. U6-RPL5A could inhibit the expression of RPL5 significantly. The growth rate of U6-RPL5A transfected cells, named AGS-U6-RPL5A, was lower than the control cells, and the percentage of the cells that was in proliferation phase was decreased by about 5%. CONCLUSION： RPL5 can inhibit the proliferation of gastric cancer cells, and further investigation of RPL5 function will be helpful in the diagnosis and treatment of gastric cancer.

关 键 词：核糖体蛋白 胃癌 SIRNA 

分 类 号：R735.2[医药卫生—肿瘤]