大肠癌中胃泌素、生长抑素mRNA的表达与细胞凋亡及Bcl-2、Bax的关系  

Relationship between expression of gastrin, somatostatin mRNA and cell apoptosis and Bcl-2, Bax in large intestinal carcinoma

作　　者：茆家定[1] 吴佩[1] 夏祥厚[1] 胡济群[1] 

机构地区：[1]皖南医学院弋矶山医院普外科,安徽省芜湖市241001

出　　处：《世界华人消化杂志》2005年第23期2757-2761,共5页World Chinese Journal of Digestology

基　　金：安徽省自然科学基金资助项目;No.03043704安徽省教育厅自然科学基金资助项目;No 2002Kj307~~

摘　　要：目的:探讨大肠癌组织中胃泌素(GAS)、生长抑素(SS) mRNA及蛋白的表达与大肠癌细胞凋亡指数(AI)和 Bcl-2、Bax的相关性．方法:采用巢式RT-PCR方法检测62例大肠癌组织中 GAS、SS的基因表达,用TUNEL法检测细胞凋亡情况, 大肠癌组织中GAS、SS、Bcl-2、Bax的蛋白表达采用免疫组织化学S-P法. 结果:大肠癌组织中GAS、SS mRNA的表达与其蛋白表达基本一致．在大肠癌组织SS高表达组、中表达组的AI明显高于SS低表达组(q=5．06,q=3．95, 均P<0．01);而在GAS各表达组中的AI变化与此相反 (q=:6．66,q=6．33,均P<0．01)．Bax、Bcl-2阳性表达率在SS和GAS低表达组、中表达组、高表达组三组间相比较存在着明显差别(x2=9．24,x2=6．91; x2=7．17,x2=13．83,均P<0．05),其中Bax在SS高表达组(80％,8／10)、中表达组(76．5％,13／17)的阳性表达率明显高于低表达组(40．0％,14／35)(x2=5．24,x2= 6．09,均P<0．05);Bcl-2与其相反(x2=4．71,x2=4．70,均 P<0．05)．Bcl-2在GAS高表达组(90．9％,10／11)、中表达组(86．7％,13／15)的阳性表达率明显高于低表达组 (44．4％,16／36)(x2=5．60,x2=7．69,均P<0．05);Bax 在GAS高表达组(27．3％,3／8)的阳性表达率明显低于低表达组(69．4％,25／36)(x2=4．59,P<0．05);而Bax 在GAS中表达组(46．7％,7／15)的阳性表达率低于低表达组,但其无明显差别．GAS／SS积分比值变化与 Bcl-2呈正相关(r=0．34,P<0．01),与AI、Bax呈负相关 (r=-0.546,P<0．01;r=-0．299,P<0．05)．结论:GAS、SS对大肠癌细胞凋亡的调控可能与 Bcl-2、Bax的异常表达有关．AIM： To explore the correlations between the expression of somatostatin （SS）, gastrin （GAS） mRNA and cell apoptosis index （AI） and Bcl-2, Bax in large intestinal cancer. METHODS： The expression of GAS and SS genes were detected in 62 colorectal cancer patients by nested reverse transcription polymerase chain reaction （RT-PCR）, and the apoptosis of the cells was detected by TUNEL method. The protein expression of Bcl-2, Bax, GAS, and SS were detected using immunohistochemical staining （S-P method）. RESULTS： The expression of GAS and SS mRNA and protein were basically consistent. The AI in SS high and moderate expression patients with large intestinal cancer was remarkably higher than that in SS low expression ones （q = 5.06, 3.95, both P 〈0.01）, while it was just opposite in GAS positive patients （q = 6.66, 6.33, P 〈0.01）. The positive rates of Bax and Bcl-2 expression had significant difference between SS （or GAS） high, moderate and low expression patients with large intestinal cancer （Bax： x^2 = 9.24, 6.91, P 〈0.05; Bcl-2： ;2 = 7.17, 13.83, P 〈0.05）. The positive rate of Bax expression in SS high （80%, 8/10） and moderate （76.5%, 13/17） expression patients was notably higher than that in the low expression ones （40.0%, 14/35） （x^2= 5.24, 6.09, P 〈 0.05）, but the rate of Bcl-2 expression was just opposite （x^2 = 4.71, 4.70, P 〈0.05）. The positive rate of Bcl-2 expression in GAS high （90.9%,10/11） and moderate （86.7%, 13115） expression patients was markedly higher than that in the low expression ones （44.4%, 16/36） （x^2 = 5.60, 7.69, P 〈0.05）, but the positive rate of Bax expression in GAS high expression patients （27.3%, 3/8） was obviously lower than that in the low expression ones （69.4%, 25/36） （x^2 = 4.59, P 〈0.05）. Bax expression was not significantly different between moderate and low GAS positive patients. The value of GAS/SS was positively correlated with Bcl-2 expression （r = 0.34, P 〈0.01）,

关 键 词：GAS、SS对大肠癌细胞凋亡的调控可能与Bcl-2 Bax的异常表达有关 

分 类 号：R735.34[医药卫生—肿瘤]