卵巢切除大鼠不同脑区胆碱乙酰化转移酶阳性细胞表达的变化(英文)  

EXPRESSION VARIETY OF CHOLINE ACETYLTRANSFERASE IN DIFFERENT BRAIN SECTIONS IN RATS WITH OVARIECTOMY

作　　者：姜长青[1] 郭宗君[2] 兰克涛[3] 金丽英[2] 杜芳[2] 

机构地区：[1]青岛市市立医院病理科,山东青岛266011 [2]青岛大学医学院脑血管病研究所 [3]青岛市第三人民医院急诊科

出　　处：《青岛大学医学院学报》2006年第1期22-24,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：SupportedbyCommitteeofScienceandTech-nologyofShandongProvince(003130110,22130109)andCommit-teeofScienceandTechnologyofQingdaoCity(SG9702-403)

摘　　要：目的 观察卵巢切除大鼠脑内不同部位胆碱乙酰化转移酶（CHAT）阳性细胞表达的变化，了解ChAT改变与轻度认知障碍（MCI）相关的可能机制。方法 成年健康雌性Wistar大鼠14只，应用SINGH法建立大鼠卵巢切除模型（模型组），对照组除不切除卵巢外，其余步骤同模型组。常规处死大鼠，取脑组织行ChAT免疫细胞化学染色，观察海马CA1区、皮质区、杏仁复合体区、基底前脑Meynert核等部位ChAT阳性细胞表达变化。结果 模型组与对照组比较脑内海马CA1区、大脑皮质区、杏仁复合体区、Meynert核区ChAT阳性细胞数明显减少（t=5．15～16．48，P〈0．01）。结论 雌激素下降可致大鼠脑内多部位ChAT阳性细胞表达减少，这种病理变化可能与MCI发病有关。Objective To observe the changes of choline acetyltransferase （CHAT） in different brain sections of rats after ovariectomy. Methods Fourteen Wistar rats were divided into two groups： model group （rats with ovariectorny）, and control group （no ovaries removed）. The brain tissues were taken out and immunoreactive cells of ChAT were observed by using immunohistochernieal technique in CA1 section of hippocarnpi, cortex section, nucleus amygdalae section and nucleus of Meynert. The amount of immunoreactive cells of ChAT was compared in different areas between the two groups. Results The immunoreactive cells of ChAT remarkably decreased in hip pocampus CA1, cerebral cortex, nucleus amygdalae and Meynert nucleus of the brain in the model group than in the control group （t=5.15- 16.48, P〈0.01）. Conclusion The reduction of estrogen in the body can cause the reduction of ChAT in some brain sections, and this pathological variation may be related to the occurrence of mild cognitive impairment.

关 键 词：胆碱O-乙酰转移酶 卵巢切除术 认知障碍 鼠 Wistar 

分 类 号：R363[医药卫生—病理学]