狼蛛优势种群的12SrRNA对长期农药胁迫的分子响应  

Molecular (12S rRNA) Response of the Wolf Spider (Araneae: Lycosidae) Populations Under Long-period Pesticide Stress

作　　者：罗育发[1] 颜亨梅[1] 

机构地区：[1]湖南师范大学生命科学学院动物系

出　　处：《应用与环境生物学报》2006年第1期55-58,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.30370208);湖南省自然科学基金项目(No.01JJY2026)资助~~

摘　　要：应用12SrRNA基因序列分析探讨了狼蛛优势种群对长期农药胁迫的分子响应机制.研究显示:(1)拟水狼蛛Piratasubpiraticus和拟环纹豹蛛Pardosapseudoannulata在长期农药胁迫下的12SrRNA基因第三结构域序列均发生了明显改变,即碱基组成由315bp和300bp分别减少为301bp和299bp,转换/颠换为17/5和11/10,核苷酸变异百分数达到0.095和0.083,同源性仅分别为85.8%和91.7%;(2)分子水平上表明拟水狼蛛较拟环纹豹蛛对农药的胁迫更为敏感;(3)供试两种狼蛛标本的基因组DNA的4个碱基对中以A和T的变异频率最高,属农药敏感型碱基对;(4)长期农药胁迫下供试两种狼蛛标本的12SrRNA基因片段的共同突变位点规律显示:192～228片段是其农药胁迫的敏感区.Molecular （ 12S rRNA） response＇s mechanisms of predominant populations of wolf spider under long-period pesticide stress were studied based on the analyzed data of mitochondrial 12S rRNA gene sequences （domain Ⅲ）. The results showed that （ 1 ） the third domain＇s sequences of mitochondrial 12S rRNA of the wolf spiders Pirata subpiraticus and Pardosa pseudoannulata were all changed obviously under long-period pesticide stress. For example, the 315 bp and 301 bp of 12S rRNA gene fell into 300 bp and 299 bp, respectively; transision/transversions were 17/5 and 11/10; the percents of nucleotide variance were 0. 095 and 0. 083 ; and homologies were only 85.8% and 91.7%, respectively. （2） It was inferred from molecular side that P. subpiraticus under long-period pesticide stress was more sensitive than P. pseudannulata. （3） The variable frequencies of A and T were highest in the 4 base pairs of DNA sequences of the two examined wolf spiders. It was clear that A and T were sensitive under long-period pesticide stress. （4） Based on the laws of base pairs＇ commonly mutated sites for 12S rRNA sequences of the two examined wolf spiders under long-period pesticide stress, the segment （192-228） was sensitive region of the base pairs＇ mutations. Fig 2, Tab 3, Ref 9

关 键 词：狼蛛优势种群 12S RRNA 序列分析 农药胁迫 

分 类 号：S476.2[农业科学—农业昆虫与害虫防治]