DG-DGGE分析除臭生物滤池微生物多样性  

Analysis of Microbial Diversity and Community Composition After Enrichment with Deodorizing Biofilter by DG-DGGE

作　　者：陈桐生[1] 李建军[2] 岑英华[2] 孙国萍[2] 

机构地区：[1]中国科学院南海海洋研究所,广州510301 [2]广东省微生物研究所

出　　处：《应用与环境生物学报》2006年第1期113-117,共5页Chinese Journal of Applied and Environmental Biology

基　　金：广东省自然科学基金团队项目(20015017);广州市科技攻关项目(2002Z10021)~~

摘　　要：以细菌的通用引物PCR扩增16SrRNA基因的V3可变区,结合应用双梯度变性梯度凝胶电泳(DGDGGE)技术分析除臭生物滤池中不同空间层次的微生物种群的基因多样性,以及富集前后的微生物种群结构变化,初步了解可培养细菌的情况,并回收主要的DNA片段进行序列分析.结果表明,在滤池的不同层次上呈现出明显的空间分布多样性差异,并且培养前后及不同培养基富集培养的微生物种群的多样性及特异性有很大的差别.序列比对显示,硫氧化细菌在除臭过程中占有优势地位,为进一步的菌种分离提供有益的指导,也为更好地处理恶臭气体提供可靠的科学支持.V3 variable fragments of genes coding for 16S rRNA were amplified by Polymerase Chain Reaction. PCR-DG-DGGE combined technique was used to analyze the microbial population composition in different spaces of deodorizing biofilter and difference in community compositions after enrichment in ordor to understand the type and quantity of culture bacteria, Then, some primary DNA fragments were recovered for sequence analysis, The results indicated that there were prominent difference spatially in microbial diversity and dramatic difference between uncultured and enriched bacteria in different media. Sequence alignment results showed that the sulfide-oxidizing bacteria were the predominant population in biofilter. Fig 4, Tab 2, Ref 18

关 键 词：除臭生物滤池 PCR DGGE 双梯度变性梯度凝胶电泳(DG-DGGE) 

分 类 号：X172[环境科学与工程—环境科学]