醛固酮合成酶基因-344C/T多态性与高血压左室肥厚相关性研究  

Relationship between aldosterone synthase(CYP11B2)-344C/T polymorphism and left ventricular hypertrophy in essential hypertension

作　　者：崔俊彪[1] 王曦云[1] 盛莉[1] 李庆祥[2] 刘艳萍[1] 张燕[1] 张淑娜[1] 

机构地区：[1]吉林大学第四医院心内科,吉林省长春市130011 [2]北京阜外医院高血压病研究室

出　　处：《中国心血管病研究》2006年第3期190-193,共4页Chinese Journal of Cardiovascular Research

摘　　要：目的观察醛固酮合成酶(CYP11B2)基因-344C/T多态性与高血压左室肥厚(LVH)的相关性。方法按心脏超声特点将314例原发性高血压患者分为左室肥厚[LVH(+)]组157例,无左室肥大[LVH(-)]组157例。使用常规方法提取白细胞DNA,采用多聚酶链式反应(PCR),限制性内切酶方法测定CYP11B2基因多态性。结果CYP11B2基因-344C/T多态性显示在LVH(+)组与LVH(-)组之间各基因型分布及等位基因频率显著不同,其中LVH(+)组TT基因型频率显著高于LVH(-)组(分别为0.66、0.51,P<0.01),而在CT基因型频率LVH(+)组显著低于LVH(-)组(分别为0.27、0.41,P<0.01);T等位基因频率LVH(+)组显著高于LVH(-)组(分别为0.79、0.72,P<0.05)。结论CYP11B2基因-344C/T多态性与高血压LVH密切相关,TT基因型频率和T等位基因频率可能是高血压LVH发生的机制之一。Objective The study was to evaluate the relationship between aldosterone synthase （CYP11B2）-344C/T polymorphism and left ventricular hypertrophy （LVH） in essential hypertension（EH）. Methods 314 patients with EH were divided into two groups on base echocardiography characteristic： the left ventricular hypertrophy [ LVH（＋ ）]group was 157 patients, and the no left ventricular hypertrophy [LVH （-）]group was 157 patients. DNA was extracted from white blood cell. Segments of CYP11B2 gene were amplified from DNA by polymerase chain reaction（PCR）.PCR products were gigested with 5U of Hae Ⅲ for 2 hours at 37℃. The digested products were subjected to electrophoresis. Results -344C/T polymorphism showed significant differentee in genotype or allele frequency distribution in the group with LVH（＋） and LVH（-）.The frequency of the -344TY genotype, T allele in LVH （＋）group was significantly higher than that in the LVH（-）group （TT respectively： 0.66,0.51,P〈0.01; T respectively： 0.79,0.72, P〈0.01）,and the frequency of the -344C/T genotype in LVH（＋） group was significantly lower than that in the LVH（-） group （CT respectively： 0.27,0.41 ,P〈0.01 ）. Conclusion The data suggest that a significant relationship between the aldosterone synthase（CYP11B2）-344C/T polymorphism and LVH in the EH could be found, the frequency of-344TT genotype and T allele may be one of mechanism in development of LVH in EH.

关 键 词：高血压 肥大 左心室 基因 多态性 限制性片段长度 细胞色素P-450 CYP11B2 

分 类 号：R544.1[医药卫生—心血管疾病]