子宫内膜癌中E-cadherin基因启动子甲基化研究  

Study on hypermethylation of E-cadherin promoter in endometrial carcinoma

作　　者：王素琴[1] 耿正惠[2] 王淑媛[1] 

机构地区：[1]山西省人民医院妇产科,030012 [2]河南郑州大学第二附属医院妇产科

出　　处：《中国妇产科临床杂志》2006年第2期116-117,138,共3页Chinese Journal of Clinical Obstetrics and Gynecology

摘　　要：目的探讨子宫内膜癌中E-cadherin基因启动子甲基化与蛋白表达的关系。方法采用甲基化特异性PCR(MSP)和免疫组化SP法检测12例正常子宫内膜、42例子宫内膜癌组织中E-cadherin基因启动子甲基化和蛋白表达。结果E-cadherin基因启动子甲基化率为35.7%,蛋白阳性率为42.9%,甲基化与蛋白表达呈负相关,二者均与癌细胞分化程度相关。结论E-cadherin基因启动子甲基化导致蛋白表达异常,参与子宫内膜癌的发生发展。Objective To study hypermethylafion of E-cadhexin promoter and the relationship of the gene modification and the gene expression in endometrial carcinoma. Methods Methylation specific PCR （MSP） and immunohistochemistry （SP） were used to detect methylation status of E-cadherin promoter and its exppression, respectively, in 12 endometrium and 42 endometrial carcinoma. Results The hypermethylation rate of E-cadherin gene was 35.7%, and the positive rate of E-cadherin was 42.9%. Both methylation and expression were related to cell differentiation. Conclusions Hypermethylation in E-cadherin promoter results in aberrant protein expression, and is involved in the development of endometrial carcinoma.

关 键 词：子宫内膜癌 E-CADHERIN 甲基化 MSP 基因启动子 

分 类 号：R737.33[医药卫生—肿瘤]