Rg1对失血性休克大鼠肠上皮细胞保护作用的实验研究  

Protective effects of Rg1 on intestinal epithelial cells in hemorrhagic shock rats

作　　者：李晓东[1] 陆松敏[1] 史成和[2] 徐华彪[3] 刘建仓[1] 龙在云[4] 李萍[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第二研究室创伤烧伤与复合伤国家重点实验室,重庆400042 [2]解放军海军总医院药剂科,北京100037 [3]解放军海军总医院海战伤研究所,北京100037 [4]第三军医大学大坪医院野战外科研究所第三研究室,重庆400042

出　　处：《创伤外科杂志》2006年第2期112-115,共4页Journal of Traumatic Surgery

基　　金：国家基础研究"973"基金资助(G1999054202)

摘　　要：目的观察失血性休克对大鼠肠黏膜组织超氧化物歧化酶(SOD)、丙二醛(MDA)、乳酸脱氢酶(LDH)、细胞线粒体膜电位、大鼠存活情况、肠上皮黏膜组织病理改变的影响,探讨人参皂苷单体Rg1对大鼠肠道损伤的保护作用及机制。方法采用失血性休克大鼠模型,紫外分光法测定各组动物处理后肠黏膜组织中SOD活性、MDA含量、LDH活力;以激光共聚焦显微镜检测肠上皮细胞线粒体膜电位的变化;观察各组动物存活情况及肠黏膜组织病理形态改变。结果失血性休克导致实验大鼠肠黏膜组织SOD活性显著降低、MDA含量显著升高、LDH活力显著降低、线粒体膜电位显著降低(P<0.01)。Rg1治疗显著提高肠黏膜组织中SOD活性、降低MDA含量、提高LDH活力;稳定肠上皮细胞线粒体膜电位(P<0.01),显著改善动物存活情况及病理形态改变。结论Rg1对失血性休克大鼠肠道上皮细胞损伤具有保护作用,其机制可能与抑制异常凋亡、抗脂质过氧化、保护线粒体功能及结构完整有关。Objective To explore the protective effects and mechanism of Rgl on intestinal epithelial cells damaged in hemorrhagic shock rats. Methods Rgl served as treatment drug, Wistar rats were divided into 4 groups： control group, hemorrhagic shock group, Rgl and resuscitation group, Rats suffered from hemorrhagic shock and maintained blood pressure 40mmHg for 2 hours, then the Rgl （5mg/kg） and saline （2 times of quantity of blood loss） were administrated in the 3 groups （except control group）. After hemorrhagic shock for 5h, SOD, MDA and LDH were determined,meanwhile MMP was detected by laser scanning eonfoeal microscopy to see if there was any difference among them. Results SOD, LDH and MMP in hemorrhagic shock group was significantly decreased, while MDA was significantly increased compared with control group （ P 〈 0.01 ）. These changes could be signifi cantly attenuated by Rgl. Conclusion Rgl has significant protective effects on intestinal epithelial cells injured by hemorrhagic shock in vivo.

关 键 词：RG1 失血性休克 肠上皮细胞 线粒体膜 电位 

分 类 号：R605.971[医药卫生—急诊医学]