非小细胞肺癌中p33^(ING1b)表达的临床及生物学意义  

The Clinical and Biological Significance for Expression of p33^(ING1b) in Non-small Cell Lung Cancer

作　　者：马华玲[1] 朱润庆[1] 曾艳[1] 刘铭球[1] 夏东[1] 肖静[1] 黄娟[1] 

机构地区：[1]武汉大学医学院病理学教研室,武汉市430071

出　　处：《中国肿瘤临床》2006年第5期241-244,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金项目资助(编号:39870305)

摘　　要：目的:探讨p33ING1b的表达与非小细胞肺癌(Non-smallCellLungCarcinoma,NSCLC)临床病理特征的关系及其在NSCLC发生、发展中的可能作用机制。方法:用免疫组化SP法检测NSCLC和非肿瘤肺组织中p33ING1b的表达及NSCLC组织p21WAF1和Bax中的表达。结果:p33ING1b在NSCLC组织中表达率显著低于非肿瘤肺组织(P<0.01)。p33ING1b低表达与肺癌的分化、分期及淋巴结转移有关。p33ING1b的表达率在低分化组(30.77%)显著低于高分化组(80.95%)、中分化组(71.43%)(P均<0.05)。在Ⅲ期和有淋巴结转移组的表达率显著低于Ⅰ～Ⅱ期和无淋巴结转移组(P均<0.01)。p33ING1b与p21WAF1表达呈正相关(P<0.01)。结论:1)p33ING1b在NSCLC中低表达,它的低表达对判断NSCLC恶性程度、浸润甚至转移有重要价值。2)p33ING1b的低表达使p21WAF1下调在NSCLC发生、发展中可能起重要作用。Objective： To explore the relationship between p33^ING1b expression and clinicopalhologic characteristics in non-small cell lung carcinoma （NSCLC） and the possible mechanism of p33^ING1b in the oncogenesis of NSCLC. Methods： hnmunohistochemistry staining （labeled streptavidir]-biolin method） were used Io detect p33^ING1b expression in NSCLC and non-neoplasia lung tissues and p21^WAF1 and Bax in NSCLC. Results： The positive rate of p33^ING1b in 61 cases of NSCLC was significantly lower than those in re）n-tumorous lung tissues, P〈0.01. The down-regulation of the expression of p33^ING1b was related to differentiation and TNM stages, as well as to lymph-node metastases. The positive rate of p33^ING1b in poorly diffrentiated group was significantly lower than those in well and moderately- differentiated group, P〈0.05. The positive rates of p33^ING1b in stage and lymph node metastases groups were significantly lower than those in stage ＋ and the groups without lymph node metastasis, P〈0.01. The expression of p21^WAF1 was positively correlated with the expression of p33^ING1b, P〈0.01. Conclusion： 1） The expression of p33^ING1b is reduced in NSCLC. The down-expression of p33^ING1b is useful for evaluating the malignancy level, invasion and metastasis of NSCLC; 2） The down-expression of p33^ING1b might allow the down-regulation of p21^WAF1 to play an important role in the oncogenesis of NSCLC.

关 键 词：肺肿瘤 p33^ING1b蛋白 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]