基于醇溶蛋白的20份小麦种质遗传完整性分析  

Genetic Integrity of 20 Wheat Accessions Based on Gliadin Analysis

作　　者：任守杰[1] 张志娥[2] 陈晓玲[2] 王建华[1] 卢新雄[2] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100094 [2]中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《植物遗传资源学报》2006年第1期44-48,共5页Journal of Plant Genetic Resources

基　　金：国家"十五"攻关项目(2004BA525B01)

摘　　要：采用醇溶蛋白电泳技术对同一品种不同繁殖年份的20份小麦种质进行遗传完整性分析。结果表明：供试种质中有10份具有一种醇溶蛋白谱带带型的同质性种质；另外10份具有2～4种醇溶蛋白谱带带型的异质性种质，其中6份为地方品种。表明地方品种具有较高的遗传多样性。在10份异质性种质中，两个繁殖年份种质之间的醇溶蛋白带型频率变化差异不显著的有5份，其第一繁殖年份的种质发芽率均高于75％，而另外5份存在显著差异的种质，第一年份的发芽率都低于66％。进一步分析表明，这10份异质性种质在两个繁殖年份之间，其发芽率差值与带型频率差值之间呈极显著正相关，相关系数为0．8665。上速结果表明，小麦更新时较高的发芽率是维持异质性种质遗传完整性的关键因素。The genetic integrity of 20 wheat accessions from different propagation times was studied by PAG-electrophoresis. The result showed that there were 10 genetically homogeneous accessions having only one gliadin spectrum biotype and 10 genetically heterogeneous accessions having 2 - 4 gliadin spectrum biotypes. Among 10 genetically heterogeneous accessions, 6 were landraces. It showed that landraces had high heredity variation. The germination rates before regeneration were higher than 75 % for the 5 genetically heterogeneous accessions in which the differences of frequency of gliadin spectrum biotypes were not significant between the two populations propagated in different years. But for other 5 genetically heterogeneous accessions, the differences of frequency of gliadin spectrum biotypes were significant between the two populations propagated in different years and their germination rates were lower than germination rate was positively correlated with difference of heterogeneous accessions, and the correlation coefficient was germination rate was the main factor for maintaining the genetic 66 %. The result also showed that the difference of the frequency of biotypes at 1% level of 10 genetically 0.8665. The result indicated that the propagation integrity of heterogeneous germplasm

关 键 词：小麦 醇溶蛋白 遗传完整性 种质资源 保存 

分 类 号：S512.1[农业科学—作物学]