人参皂甙对β淀粉样蛋白诱导THP-1单核细胞一氧化氮合酶和一氧化氮的影响  

Effects of Ginsenoside on Expression of Nitric Oxide Synthase and Concentration of Nitric Oxide in THP-1 Cells Induced by β-amyloid

作　　者：李玮[1] 李林[1] 褚燕琦[1] 艾厚喜[1] 叶翠飞[1] 童萼塘[2] 孙圣刚[2] 

机构地区：[1]首都医科大学宣武医院药物研究室,北京100053 [2]华中科技大学同济医学院附属协和医院神经内科,武汉430022

出　　处：《中国神经免疫学和神经病学杂志》2006年第2期94-97,共4页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家重点基础研究发展计划-"973"基金资助项目(G2000057010;2003CB517104);国家自然科学基金资助项目(30472184);北京市自然科学基金资助项目(7032013);北京市中医药重点学科基金资助项目(2001)

摘　　要：目的研究人参皂甙对β淀粉样蛋白(βamyloid,Aβ)1-40诱导THP-1细胞表达诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和一氧化氮(nitric oxide,NO)的影响。方法用Western blotting测定THP-1细胞iNOS表达,以Griess法测定细胞上清中NO的浓度。结果Aβ模型组iNOS的表达明显高于对照组(P<0.01)。与模型组比较,人参皂甙可明显减少THP-1细胞iNOS的表达和NO的产生(P<0.05)。随着人参皂甙剂量的增大,人参皂甙可引致THP-1细胞iNOS的表达和NO的产生明显减少(P<0.05),且有剂量依赖性。结论人参皂甙可抑制THP-1细胞iNOS的表达,进一步影响NO的产生。Objective To investigate the effects of ginsenoside （GS） on expression of inducible nitric oxide synthase （iNOS） and concentration of nitric oxide （NO） in THP-1 cell line induced by β-amyloid （Aβ）1-40. Methods The concentration of nitric oxide was determined by Griess reagent in supernatant of THP-1 cells induced by Aβ. The content of iNOS was measured by Western blotting. Results expression of iNOS and concentration of NO in Aβ model group were significantly higher than those in control group, while the GS treatment reduced expression of iN（）S and concentration of NO obviously in THP-1 cells induced by Aβ. Compared with model group, expression of iNOS and concentration of NO were remarkably lower. Ginsenoside causes dose-dependent decrease in expression of iNOS and concentration of NO. Conclusions GS significantly inhibited expression of iNOS and reduced secretion of NO in THP-1 monoeytes induced by Aβ. GS might provide a novel therapy for AD.

关 键 词：阿尔茨海默病 人参皂甙 Β淀粉样蛋白 诱导型一氧化氮合酶 一氧化氮 

分 类 号：R749.16[医药卫生—神经病学与精神病学]