白细胞介素-2基因佐剂对单纯疱疹病毒糖蛋白D DNA疫苗的免疫增强作用  

Immune Enhancing Effect of Interleukin-2 Gene Adjuvant on HSV-Ⅰ gD DNA Vaccine

作　　者：李伟荣[1] 王得新[1] 杨琪[2] 王健伟 王佳伟[1] 冯子敬[1] 

机构地区：[1]首都医科大学附属北京友谊医院神经内科,北京100050 [2]首都儿科研究所,北京100083 [3]中国疾病预防与控制中心病毒病预防控制所,北京100052

出　　处：《中国神经免疫学和神经病学杂志》2006年第2期98-101,共4页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的探讨白细胞介素-2(IL-2)基因佐剂对单纯疱疹病毒Ⅰ型(HSV-Ⅰ)糖蛋白D(gD)DNA疫苗的免疫调节作用。方法将表达HSV-ⅠgD基因的DNA疫苗质粒IRES-gD以及HSV-ⅠgD与IL-2基因共表达的重组质粒IRES-gD-IL-2分别注入BALB/c鼠股四头肌,检测特异性抗体和中和抗体的产生情况,并于第3次注射后14 d用滴度100组织培养半数感染量(TCID50)的HSV-1病毒感染小鼠,观察小鼠的生存情况。结果IRES-gD组和IRES-gD-IL-2组均可刺激小鼠产生抗HSV-ⅠgD的特异性抗体和中和抗体,抗体滴度随免疫次数增加而升高,IRES-gD-IL-2组小鼠产生的抗体及中和抗体滴度明显高于IRES-gD组,两组小鼠的生存率差异无统计学意义。结论IL-2基因佐剂可促进HSV-ⅠgD DNA疫苗诱导抗体的产生,并具有免疫增强作用。Objective To investigate the regulating effect of interleukin-2 （IL-2） gene on the immune response induced by Herpes Simplex virus type [ （HSV-Ⅰ） glycoprotein D （gD） gent in mice. Methods BALB/C mice were inoculated intramuscularly with IRES-gD which encoded HSV-Ⅰ gD gene and co-expressive nucleic vaccine plasmid of IRES-gD-IL-2, which containing both HSV-Ⅰ gD and IL-2 gone. Sera were collected for measuring IgG antibody and neutralizing antibody, and the number of surviving animals was monitored up to 14 days afterHSV-Ⅰ infection, Results Mice vaccined with IRES-gD-IL-2 had significantly higher IgG antibody titer and neutralizing antibody titer than that seen in IRES-gD group. But the surviving rate of animals showed no difference in the two groups. Conclusions Injection of mice with plasmid DNA co-expression of HSV-Ⅰ gD gene and IL-2 gene could enhance immune responses efficiently.

关 键 词：单纯疱疹病毒 糖蛋白D 白细胞介素-2 DNA疫苗 

分 类 号：R744[医药卫生—神经病学与精神病学]