外源基因异常分离的抗除草剂水稻9311HR bar基因的遗传传递  

Heredity of Abnormal Segregation Foreign Gene bar in Herbicide Resistant Rice 9311HR

作　　者：林静[1] 张亚东[1] 朱镇[1] 赵凌[1] 陈涛[1] 张所兵[1] 王才林[1] 

机构地区：[1]江苏省优质水稻工程技术研究中心暨江苏省农业科学院粮食作物研究所

出　　处：《江苏农业学报》2006年第1期1-4,共4页Jiangsu Journal of Agricultural Sciences

基　　金：国家863项目(2003AA207020);江苏省高技术研究计划项目(BG2001304;BG2004304;BG2005301);江苏省植物功能基因组学重点实验室开放课题(02738800338)

摘　　要：在以基因枪转化获得的抗除草剂转bar基因材料HR为供体亲本,9311为受体亲本的回交转育过程中,发现回交转育后代9311HR抗性单株自交后代中bar基因始终表现1∶1的异常分离。以9311HR抗性单株作母本,与常规非转化品种杂交获得的F1,除草剂抗性植株和非抗性植株比例为1∶1;以9311HR抗性单株作父本,与常规非转化不育系的杂交后代均不抗除草剂。PCR分析结果显示,F1抗性植株PCR扩增呈阳性,非抗性单株PCR扩增呈阴性。上述结果表明,9311HR中外源基因bar仅能通过雌配子传递给后代,而不能通过雄配子即花粉传递给后代。推测HR中bar基因的插入位点可能与某一育性相关基因紧密连锁。Abnormal segregation of foreign gene bar was observed in a herbicide resistant rice 9311HR developed by transferring the bar gene from the donor, a genetically modified organism （GMO） H R obtained by biolistic method, into the breeding line 9311 by backcrossing. In order to know the heredity transmission of bar gene in the progeny, the herbicide resistant plant segregated from 9311HR was used as female or male to cross with conventional rice variety or CMS line, and the segregation ratio of herbicide resistant to sensitive plants was investigated in the test F1 population. When the resistant plant was used as female parent,the segregation ratio of resistant to sensitive plants was 1： 1. However, when it was used as male parent. all the progenies were sensitive to herbicide. Result of PCR analysis indicated that bar zane amplification of the herbicide resistant plants were positive and the herbicide sensitive plants were negative. The biological and genetic basis of distorted segregation of bar gene was discussed and deduced on the basis of above results, and the authors are inclined to ascribe these phenomena to the absent transmit ability of 9311HR pollens.

关 键 词：水稻 BAR基因 异常分离 遗传 

分 类 号：S511.210.353[农业科学—作物学]