多器官功能不全综合征患者中性粒细胞凋亡规律与前列腺素E1的关系  

The change of apoptosis of polymorphonuclear neutrophil (PMN) in patients with multiple organ dysfunction syndrome(MODS) after severe trauma and the effect of Prostaglandin E1(PGE1)

作　　者：陈大庆[1] 朱烈烈[1] 林露阳[1] 

机构地区：[1]温州医学院附属第二医院急诊科,温州325027

出　　处：《中华急诊医学杂志》2006年第3期252-254,共3页Chinese Journal of Emergency Medicine

摘　　要：目的观察外伤后多器官功能不全综合征(MODS)患者外周血中性粒细胞(PMN)凋亡规律及其受前列腺素E1(PGE1)的影响。方法应用AO/EB荧光染色法检测并比较外伤后MODS患者组(40例)、外伤后无MODS患者组(20例)及正常人组(20例)外周血体外培养PMN凋亡率的差异。同时观察不同浓度PGE1在不同作用时间内对PMN凋亡的影响。结果外伤后MODS患者组外周血PMN凋亡率明显降低,加入PGE1后PMN凋亡率明显提高(P<0.05)。结论外伤后MODS患者存在PMN凋亡滞后现象,PGE1能够加速外伤后MODS患者PMN凋亡。Objective To investigate the change of apoptosis of polymorphonuclear neutrophil （PMN） in patients with multiple organ dysfunction syndrome （MODS） after severe trauma and the effect of Prestaglandin E1 （PGE1）, Methods AO/EB fluorescent staining method was used to detect the apoptosis ratio of PMN in 40 patients with MODS and 20 patients without MODS after severe trauma, and 20 healthy people （control group）. Different concentration of PGE1 was added to the all patients and its effect on PMN was observecl. Results Apoptosis ratio of PMN decreased significantly in patients with MODS and increased when PGE1 was added （P 〈 0.05）, PGE1 increased the level of apoptosis of PMN. Conclusion After severe trauma, patients with MODS have PMN apoptosis hysteresis, Apoptosis ratio of PMN in these patients significantly decreases and could be amended by PGE1.

关 键 词：多器官功能不全综合征 中性粒细胞 凋亡 前列腺素 

分 类 号：R365[医药卫生—病理学]