胃癌患者EB病毒感染对bcl-2基因表达影响的研究  

Influence of EB virus infection to bcl-2 expression in gastric carcinoma patients

作　　者：李淑英[1] 侯灵彤[2] 周天戟[1] 

机构地区：[1]华北煤炭医学院生物系,河北唐山063000 [2]华北煤炭医学院附属医院妇产科,河北唐山063000

出　　处：《临床荟萃》2006年第5期319-321,F0002,共4页Clinical Focus

基　　金：教育部留学回国人员科研启动基金(编号教外司留[2000]479)

摘　　要：目的研究胃癌患者癌组织中人疱疹病毒4型(EB病毒,epstein-Barr virus,EBV)感染与B细胞淋巴瘤或血血病-2基因(B cell lymphoma/leukemia-2,bcl-2)表达的关系。方法原位杂交法(In Situ Hybridization,ISH)检测胃癌患者石蜡标本中EBV编码的小RNA(EBER1);免疫组化法(immunohistochemistry,IHC)检测EBV阳性胃癌及EBV阴性胃癌标本癌基因bcl-2的表达状况。结果217例胃癌患者癌组织标本中检测到23例呈EBER1阳性;EBV阳性胃癌组Bcl-2蛋白表达显著高于EBV阴性胃癌组,平均面积(avevage area,AA)、平均光密度(mean opticaldensity,MOD)和积分光密度(integral optical density,IOD)分别为(67.31±5.95)μm2、17.60±2.80和1.75±0.13 vs(38.1±4.14)μm2、5.000.0和1.35±0.07(P<0.01)。结论EBV感染可能上调了癌基因bcl-2表达。Objective To investigate the influence of EB virus（EBV） infection on the expression of oncogene bcl-2. Methods EBER1 in 217 cases of gastric carcinoma were detected with in situ hybridization. Then, EBV positive and negative cases were selected for analysis of the expression of bcl-2 by immunohistochemistry. Results In 217 eases of gastric carcinoma, there were 23 cases with EBER1 positive. The expression of bcl-2 was higher in EBV positive gastric carcinoma than that in EBV negative, respectively AA, MOD and IOD（67.31 ± 5.95）μm^2 , 17.60 ± 2.80 and 1.75±0.13 vs （38.1±4.14）μm^2,5.000.0 and 1.35±0.07（P 〈0.01）. Conclusion EBV infection in gastric carcinoma increased the expression of bcl-2.

关 键 词：疱疹病毒4型 人 胃肿瘤 癌基因蛋白质类 

分 类 号：R735.2[医药卫生—肿瘤]