天癸更年软胶囊对老年大鼠卵巢颗粒细胞凋亡的影响  

Effects of Tiangui menopause capsules on rat ovarian granulosa cell apoptosis

作　　者：赵丽梅[1] 袁秉祥[1] 魏霞蓁[2] 胡锐[1] 刘波[1] 康军[2] 陈东[2] 

机构地区：[1]西安交通大学医学院药理学教研室,陕西西安710061 [2]西安交通大学天奎医药生物公司,陕西西安710061

出　　处：《中国药理学通报》2006年第3期317-320,共4页Chinese Pharmacological Bulletin

基　　金：国家十五重大科技专项"创新"药物和中药现代化"863"专项基金资助项目(No2003AA2Z3265)

摘　　要：目的通过对老年大鼠卵巢颗粒细胞凋亡及相关基因bc l-2、fas表达的观察,探讨天癸更年软胶囊所含沙棘脂肪酸对围绝经期女性卵巢功能的调节作用,推测沙棘脂肪酸治疗围绝经期综合征的作用机制。方法采用22个月龄的SD♀大鼠,随机分为老年对照组、雌激素组、沙棘脂肪酸大、中、小剂量组,另设青年大鼠对照组。用TUNEL法研究各组大鼠卵巢颗粒细胞凋亡情况,采用免疫组化法观察bc l-2及fas蛋白表达情况和原位杂交方法检测bc l-2 mRNA及fas mR-NA表达情况,用Q55OCW图像分析系统进行灰度分析。结果大中小剂量组均可不同程度抑制卵巢颗粒细胞凋亡,增强bc l-2蛋白及mRNA表达,减弱fas蛋白及mRNA表达。结论沙棘脂肪酸通过调整bc l-2和fas表达,抑制卵巢颗粒细胞凋亡,从而实现调节雌激素水平,改善围绝经期症状。Aim To observe effects of Seabuckthorn fatty acids on old rat ovarian granulosa cell apoptosis and expression of bcl-2 and fas, then discuss the adjustive effect of Seabuckthorn fatty acids on ovarian function of menopause. Methods 22 months old rats were randomly divided into 5 groups, estrogen control group, old control group, Seabuckthorn fatty acids group of high dose, middle dose and low dose. In addition, one young control group was made up of 3 months old rats. Rat ovarian granulosa cell apoptosis was detected by TUNEL. The expression of protein bcl-2 and protein fas were detected by immunohistochemistry, and bcl-2 mRNA and fas mRNA by in situ hybridization （ISH）. Grey analyse was done using Q550CW image analyse system. Results Compared with the old rats control group, Seabuckthorn fatty acids of high dose, middle dose and low dose can differently restrain rat ovarian granulosa cell apoptosis, enhance the expression of protein bcl-2 and bcl-2 mRNA, weakened the expression of protein fas and fas mRNA. Conclusion Seabuckthorn fatty acids restrains ovarian granulosa cell apoptosis through adjusting the expression of bcl-2 and fas. Sequentially, it adjusts estrogen level and treats menopause syndrome.

关 键 词：沙棘脂肪酸 天癸更年软胶囊 颗粒细胞 凋亡 免疫组化 原位杂交 

分 类 号：R-332[医药卫生] R282.71