人牙周膜细胞体外培养和机械压力模型构建  

Culture of Human Periodontal Ligament Cells and Establishment of Mechanical Pressure Model in Vitro

作　　者：张建兴[1] 黄生高[1] 钟孝欢[1] 熊培颖[1] 

机构地区：[1]中南大学湘雅二医院口腔科,湖南长沙410011

出　　处：《口腔医学研究》2006年第1期38-42,共5页Journal of Oral Science Research

摘　　要：目的:培养人牙周膜细胞(hum an periodontal ligam ent cells,HPDLCs)获得HPDLCs有限细胞系,建立简单易行的HPDLCs持续静压力(continuously compressive pressure,CCP)模型。方法:组织块酶消化湿性贴壁法原代培养HPDLCs,传代并鉴定细胞来源、观察细胞生长情况,绘制4代细胞生长曲线。用圆形玻璃片给单层细胞接触加压,对细胞施加1 g/cm2、2 g/cm2、3 g/cm2、4 g/cm248 h,用MTT法检测加压对细胞的影响。结果:成功获得HP-DLCs有限细胞系,加压后当力值小于4 g/cm2时细胞无明显受损,力值增大到4 g/cm2时,细胞出现损伤。结论:组织块酶消化湿性贴壁法可以提高HPDLCs原代培养成功率,适宜力值的玻片直接接触加压模型可初步模拟压力对牙周膜细胞的生物学作用。Objective： To obtain a finite cell line of human periodontal ligament cells （HPDLCs） , and to establish a pressure model of HPDLCs. Methods： The primary HPDLCs were isolated from human periodontal ligament by explants with enzymatic digestion. We transfered and identificated the origin of the cells, observed the status of cell growth, and drew a growth curve of the 4th generation ceils. Ceils monolayer was compressed directly by round glass sheet with 1 g/ cm^2, 2 g/cm^2, 3 g/cm^2 and 4 g/cm^2 for 48hr, then monitored the damage of cells by MTT - method. Results： A HPDLCs finite cell line was successfully established. HPDLCs was not obviously damaged after compressed when the pressure was less than 4 g/cm and cells was markedly destroyed when the pressure was raised to 4 g/cm^2. Conclusion： The method of cell culture by explants with enzymatic digestion enhances successful - rate of primary culture of HPDLCs. The model with suitable pressure can approximately imitate the biological effect of HPDLCs under pressure.

关 键 词：牙周膜细胞 细胞培养 压力 细胞体外培养 机械压力 模型构建 

分 类 号：R783.5[医药卫生—口腔医学]