黑曲霉mnn9基因缺失株的构建及其功能分析  

Construction and functional analysis of the mnn9 gene disruption mutant in Aspergillus niger

作　　者：王永超[1] 秦浚川[1] 王敖全[2] 唐国敏[2] 王华明 

机构地区：[1]南京大学生命科学院 [2]中国科学院微生物研究所真菌和地衣系统学开放实验室 [3]Genencor International Incorporation,California94304,USA

出　　处：《菌物学报》2006年第1期70-76,共7页Mycosystema

基　　金：Joint Project granted by Genencor International Inc.of USA

摘　　要：本研究通过分析比较黑曲霉基因组与酿酒酵母基因组序列同源性,分离鉴定了黑曲霉mnn9基因。通过同源重组,在黑曲霉GICC2773(ΔAP4:pGPT-laccase)菌株中敲除了mnn9基因。该黑曲霉mnn9基因缺失使外源蛋白漆酶的分泌表达提高了14%,内源蛋白葡萄糖淀粉酶的分泌表达则降低了4%。Here we use a comparative genomics approach to isolate mnn9 gene from Aspergillus niger which share high homology with S. cerevisiae mnn9 encoding mannosyltransferase responsible for the majority of the a-1,6-polymerizing activity of the mannoprotein . By double crossover, mnn9 gone in Aspergillus niger GICC2773 （△AP4： pGPT-laccase） strain was knocked out. Like all members of the S. cerevisiae mnn9 disruption mutant, in Aspergillus niger the mnn9 gene disruption mutant enhances heterologous protein laocase production by about 14% as compared with the parental strains, but decreases homologous protein glucoamylase production by about 4%.

关 键 词：丝状真菌 同源重组 外源蛋白分泌 

分 类 号：Q78[生物学—分子生物学]