原生质体技术选育桃红侧耳优良菌株  

Applied studies on selection of high quality Pleurotus djamor strains by protoplast technology

作　　者：王谦[1] 刘玉霞[1] 张渊[2] 王士奎[3] 齐玲娣[1] 

机构地区：[1]河北大学生命科学学院,保定071002 [2]保定师范专科学校生物系,保定071051 [3]农业部规划设计研究院,北京100026

出　　处：《菌物学报》2006年第1期83-87,共5页Mycosystema

基　　金：河北省生物工程重点学科资助

摘　　要：研究了桃红侧耳原生质体的制备及再生,并进行了原生质体再生株的筛选工作。实验表明,培养5天的桃红侧耳菌丝体30℃酶解3h原生质体数目可达8.4×107/mL。原生质体再生率在1号再生培养基上最高,为6.9%。再生菌株经筛选后,得到长速显著快于出发菌株的优良菌株H120和H247。经F3代栽培实验证明:H120和H247的生物学效率明显优于出发菌株,且差异极显著。酯酶同工酶分析表明:H120和H247酶谱均发生了变化。The preparation and regeneration of protoplast from Pleurotus djamor and the screening of protoplast regenerating strains were studied. Under the conditions as 30℃ 3h enzymdysis, the number of protoplasts of P. djamor mycelial aged 5 d could reach 8.4 × 10^7/mL. The highest regeneration rate of protoplast was 6.9% in the No.1 medium. Obtaining from the screening of protoplast regenerating strains, the strains H120 and H247 showed significantly rapid growth rate as compared with the original swain. The biological efficiency of H120 and H247 （F3 generation） were higher than that of original swain at significant difference level.Esterase isoenzyme analysis results showed that the variation existed among H120, H247 and original swain in zymogram of esterase isoenzyme.

关 键 词：原生质体再生率 生物学效率 菌株选育 

分 类 号：S646[农业科学—蔬菜学]