壳聚糖固定β-半乳糖苷酶的研究  

Study on the β-Galactosidase was Immobilized with Chitcsan

作　　者：曾杨清[1] 

机构地区：[1]四川工商职业技术学院,四川都江堰611830

出　　处：《四川食品与发酵》2006年第1期32-37,共6页Sichuan Food and Fermentation

摘　　要：以壳聚糖微球为载体,戊二醛为交联剂,固定β-半乳糖苷酶,对β-半乳糖苷酶的固定化条件及固定化酶的各种性质进行了研究,确定了酶固定的最适条件为:用pH6.5的P-E-M缓冲液浸泡10h,25℃壳聚糖微球与0.5%戊二醛交联12h以上,4℃下酶与壳聚塘微球固定12h以上酶活力回收可达67%。固定化酶的最适温度为40℃左右,最适pH7.0。通过双倒数求回归方程,求得动力学参数Km值为0.613μmol/ml。固定化酶稳定性好,可以重复使用。β-Galactosidase was immobilized on chitosan microspheres with glutaraldehyde by cross - linking reaction,the immobilezation conditions and characterization of the immobilized enzyme were studide. The optimal conditions for immobilization were as follows： in pH 6.5 P -E -M solution for 10 h, chitosan microsphere was treated with 0. 5% glutaraldehde at 25℃ for 12h,the solution of β- Galactosidase was immobilized on the carrier at 4℃ for 12h, enzyme activity recovery was 67%. Optimal temperature and pH for the immobilized enzyme were about 40℃ and 7.0 respectively. Apparent michealis constant of immobilized β-- Galactosidase Km = 0. 613 mmol/L. The stability of immobilized β- Galactosidaze was high after storage.

关 键 词：壳聚糖 微球 固定化 Β-半乳糖苷酶 

分 类 号：TQ92[轻工技术与工程—发酵工程]