酪丝亮肽对人肝癌BEL-7402细胞的抑制作用及对NK细胞杀伤活性的影响  

Inhibitive Effect of Tripeptide Tyroserleutid(YSL)on Human Hepatocellular Carcinoma BEL-7402 cells and Its Effect on the Activity of NK Cell

作　　者：陆融[1] 王松[1] 赵琳[1] 贾静[1] 李会强[1] 赵鹏鹏[1] 李国立[1] 傅正[1] 姚智[1] 

机构地区：[1]天津医科大学免疫教研室

出　　处：《中国肿瘤》2006年第3期187-189,共3页China Cancer

基　　金：国家"863"项目(2004AA2Z3170;2005AA2Z3D40);国家重点基础研究发展规划(973)前期专项(2003CCA04300);教育部重点项目(03007)

摘　　要：[目的]研究三肽化合物酪丝亮肽(tyroserleutide,YSL)对人肝癌BEL-7402裸鼠移植瘤的抑制作用及对自然杀伤细胞(NK细胞)杀伤活性的影响。[方法]建立人肝癌BEL-7402裸鼠移植瘤模型,观察YSL对肿瘤生长的抑制作用;采用四甲基偶氮唑蓝(MTT)法检测YSL对正常小鼠及人肝癌BEL-7402荷瘤鼠的NK细胞杀伤活性的影响。[结果]YSL[160μg(/kg·d)]能抑制BEL-7402的生长,肿瘤抑制率为72.23%,且能增强BEL-7402荷瘤裸鼠及正常小鼠NK细胞杀伤活性,与生理盐水组相比均具有显著性差异(P<0.05)。[结论]YSL对人肝癌BEL-7402裸鼠移植瘤有抑制作用,并具有增强正常及荷瘤小鼠NK细胞杀伤肿瘤细胞活性的作用。[Purpose lTo investigate the inhibitive effect of tfipeptide tyroserleutide（YSL） on human hepatocellular carcinoma BEL-7402 transplanted tumor in nude mice and its effect on the activity of natural killer cells （NK cell）. [Methods I Human hepatocellular carcinoma BEL-7402 transplanted tumor model in nude mice was created.The inhibition of YSL on tumor growth was studied.The effect of YSL on the activity of NK cell in normal mice and in nude mice transplanted with hepatocellular carcinoma BEL-7402 were detected by methyl thiazolyl tetrazolium （MTI＇） eolofimetfic assay. [Results]YSL [160μg/ （kg·d）] could inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,with tumor inhibition rate 72.23%,and could enhance the activity of NK cell in natural mice and in nude mice transplanted with hepatocellular carcinoma BEL-7402,which all had significant difference compared with the control group （P 〈0.05）. [Conclusions ]YSL has inhibition effect in hepatocellular carcinoma BEL-7402 transplanted tumor, and can enhance the activity of NK cell.

关 键 词：酪氨酸 丝氨酸 亮氨酸 小鼠 裸 肿瘤 实验性 肝肿瘤 杀伤细胞 天然 

分 类 号：R73-3[医药卫生—肿瘤]