疏解少阳和祛风止痛法对大鼠体外血栓形成及血小板聚集功能的影响(英文)  

Effects of the methods of treating Shaoyang by dispersing and dispelling wind and relieving pain on thrombosis in vitro and function of platelet aggregation in rats

作　　者：李冀[1] 旺建伟[1] 

机构地区：[1]黑龙江中医药大学基础医学院,黑龙江省哈尔滨市150040

出　　处：《中国临床康复》2006年第7期171-173,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:偏头痛病位多在少阳经循经之处,以疏解少阳、祛风止痛法从少阳胆经治疗偏头痛疗效显著,但该两法对体外血栓形成及血小板聚集功能的作用又如何呢?目的:通过大鼠体外血栓形成实验,大鼠血小板聚集功能实验,探讨疏解少阳法与祛风止痛法对两者的影响,并通过拆方配伍实验,探讨疏解少阳法与祛风止痛法的配伍意义。设计:完全随机分组设计,对照实验。单位:黑龙江中医药大学基础医学院方剂学教研室。材料:实验于2000-03/08于黑龙江中医药大学方剂学方药实验室完成。选用健康成年Wistar大鼠60只。随机将大鼠分为6组:正常对照组、阳性对照组,全方高剂量组,全方低剂量组,疏解少阳组,祛风止痛组,每组10只。方法:①阳性对照组:按0.39g/kg剂量灌胃0.034g/mL复方羊角片(哈尔滨生物化学制药二厂生产)混悬液;全方(全方剂成分:柴胡20g,黄芩10g,半夏15g,甘草10g,川芎20g,天麻15g,细辛5g,全蝎5g,蜈蚣5g)高、低剂量组:按17.40,8.70g/kg剂量灌胃1.5,0.75g/mL全方剂;疏解少阳组(疏解少阳剂成分:柴胡20g,黄芩10g,半夏15g,甘草10g)和祛风止痛组(祛风止痛剂成分:川芎20g,天麻15g,细辛5g,全蝎5g,蜈蚣5g):按8.70g/kg剂量灌胃0.75g/mL疏解少阳剂和祛风止痛剂;正常对照组:灌胃等体积生理盐水。各组连续干预12d。②体外血栓形成实验:末次给药后2h,分离一侧颈总动脉。结扎远心端,并在近心端以动脉夹阻断血流,将动脉插管插入颈总动脉内。松开动脉夹,取1.8mL血液入一血栓形成仪的旋转环内,以17r/min旋转15min。停止转动后,倒出形成的血栓,测量血栓长度、血栓湿重。将湿血栓烘干,测量血栓干重。③血小板聚集功能测定:采用比浊测定法记录富血小板血浆在1,5min的聚集率,5min内最大聚集率及聚集抑制率。④计量资料比较采用t检验。主要观察指标:疏解少阳法和祛风止痛法对体外血栓形成及血小�BACKGROUND： Migraine is usually located in the course of Shaoyang channel, and the curative effect of treatment with methods of treating Shaoyang disease by dispersing and dispelling wind （DW） as well as relieving pain （RP） on migraine is prominent, but how about the effects of these two ways on thrombogenesis in vitro and the function of platelet aggregation （PA）？ OBJECTIVE： To probe into the effect of treatment of Shaoyang disease and methods of DW and RP on thrombogenesis in vitro and function of PA through＇the experiments of thrombogencsis in vitro and function of PA in rats, and investigate the compatible significance of DS and DW as well as RP through formula compatible experiment. DESIGN： Completely randomized grouping design and controlled trial. SETTING： Department of Prescription, College of Basic Medical Sciences, Heilongjiang University of Chinese Medicine. MATERIALS： The experiment was conducted in the Laboratory of Department of Prescription, Heilongjiang University of Traditional Chinese Medicine from March to August 2000. Sixty heahhy aduh Wistar rats were randomly divided into 6 groups： Normal control group, Shaoyang treatment group （groupⅠ ）, DW and RP group （group Ⅱ ）, high-dose and whole-prescription group （group Ⅲ）, low-dose and whole-prescription group （group Ⅳ）, and positive control group with 10 rats in each group. METHODS： ① Positive control group： Gastric perfusion of 0.034 g/mL compound divaside slice suspension （made by Harbin Second Biochemical Pharmacy co., Ltd） was given to rats （0.39/kg）; Group Ⅲ and IV （ingredients： Chaihu 20 g, huangqin 10 g, banxia 15 g, gancao 10 g, chuanqiong 20 g, tianma 15 g, xixin 5 g, quanxie 5 g, wugong 5 g）： Gastric perfusion of 1.5, 0.75 g/mL of complete prescription was given to rats （17.40, 8.70 g/kg）; Group Ⅰ （ingredients： Chaihu 20 g, huangqin 10 g, banxia 15 g, gancao 10 g） and group Ⅱ （ingredients： Chuanqiong 20 g, tianma 15 g, xixin 5 g, quanxie 5 g, wug

关 键 词：止痛剂(中药)/治疗应用 血栓形成/中药疗法 血小板聚集 

分 类 号：R364.1[医药卫生—病理学]