水飞蓟宾对抗大鼠酒精性脂肪肝改善肝功能的作用(英文)  

Effects of silibinin in improving liver function of rats with alcoholic fatty liver

作　　者：梁基智[1] 林洁茹[1] 谭海荣 吴谦[3] 潘竞锵 肖柳英 韩超 郑琳颖[1] 李博萍[1] 

机构地区：[1]广州市中医医院药剂科,广东省广州市510130 [2]广州市中医中药研究所药理研究室,广东省广州市510130 [3]广东省药物研究所药理研究室,广东省广州市510180

出　　处：《中国临床康复》2006年第7期183-185,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省医学科学技术研究基金项目(A2004546);广州市中医药,中西医结合课题资助项目(200237)~~

摘　　要：背景:水飞蓟宾(素)可产生抗自由基活性、抗脂质过氧化、抗脂氧酶、抗还原性谷胱甘肽排空、抗肿瘤以及降血脂等广泛的药理效应。在临床上,水飞蓟宾也常用于酒精性肝病的治疗。目的:探讨水飞蓟宾对大鼠酒精性脂肪肝作用的药理学机制。设计:随机对照实验。单位:广州市中医医院。材料:实验于2003-08/10在广东省药物研究所无特殊病原菌级动物实验室完成,选用无特殊病原菌级SD大鼠57只,雌雄各半,体质量(150±10)g。益肝灵片主要成份为水飞蓟宾,38.5mg/片,湖南省株洲市制药三厂生产。方法:在57只SD大鼠中随机选出18只作为正常对照组,给予生理盐水灌胃,饮用蒸馏水代替乙醇,普通饲料喂养10周。其余大鼠自由饮用100mL/L乙醇,加高脂高热量饲料喂养6周,构建大鼠酒精性脂肪肝模型,经相关指标检测确定模型成立。将剩余大鼠随机分成模型对照组18只和水飞蓟宾组21只,模型对照组给予蒸馏水灌胃,水飞蓟宾组给予水飞蓟宾100mg/kg。同时继续给予100mL/L乙醇和高营养饲料,4周后在麻醉状态下处死大鼠,取大鼠肝块进行病理观察,测定三酰甘油、超氧化物歧化酶、还原性谷胱甘肽和丙二醛水平。主要观察指标:大鼠血清中天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶活性和三酰甘油、胆固醇、低密度脂蛋白-胆固醇、高密度脂蛋白-胆固醇、大鼠肿瘤坏死因子α、大鼠转化生长因子β1的含量。结果:57只无特殊病原菌级SD大鼠全部纳入结果分析。水飞蓟宾能够抑制病鼠血清天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶活性(2550.5±400.1),(533.4±100.0),(2217.1±750.2)nkat/L,与模型组比较(3600.7±666.8),(800.2±100.0),(2900.6±1333.6)nkat/L,差异有显著性意义(P<0.05～0.01);水飞蓟宾组三酰甘油、低密度脂蛋白-胆固醇、大鼠肿瘤坏死因子α和大鼠转化生长因子β1含量(1.8±0.8),BACKGROUND： Silibinin has broad pharmaceutical effects, such as anti-free radicals, anti-lipid peroxidation, anti-lipoid oxidase, anti-glutathione （GSH） depletion, anti-neoplastie and serum lipid-lowering effects. Clinically, silibinin is often used in treating alcoholic liver disease. OBJECTIVE： To investigate the pharmacological mechanism of silibinin for alcoholic fatty liver in rats. DESIGN： Randomized and controlled study. SETTING： Guangzhou Hospital of Traditional Chinese Medicine. MATERIALS： The experiment was conducted at the Animal Experimental Laboratory of Guangdong Pharmaceutical Institute from August to October 2003. Tαally 57 SD rats, without unusual bacteria, weighting （150±10） g and of either gender, were selected. Yiganling tablets containing 38.5 mg silibinin were produced by Zhuzhou No.3 Pharmaceutical Factory （Batch No. 20020808）. METHODS： Among the 57 SD rats, 18 rats were regarded as normal control group. Rats in normal control group were administered with normal saline by gavage, and fed with normal food and distilled water in place of alcohol for 10 weeks. Rats in model group and silibinin group were fed with high-calorie food and 100 mL/L alcohol for 6 weeks to establish model of rat alcoholic fatty liver. The αher rats were divided into model control group （n=18） and silibinin group （n=21）. Rats in model control group were treated with distilled water while those in silibinin group were treated with 100 mg/kg silibinin. Meanwhile, 100 mL/L ethanol and hyperalimentation feed were given for 4 weeks. After animals were killed, TG, SOD, GSH and MDA levels were measured with liver suspension. MAIN OUTCOME MEASURES： Contents of serum alanine aminαransferase （ALT）, aspartate aminαransferase （AST）, alkaline phosphatase （AKP）, tαal cholesterol （TC）, triacylglycerol （TG）, low-density lipoprαein cholesterol （LDL-C）, high-density lipoprαein cholesterol （HDL-C）, tumor necrosis factor （TNF）-α, and transforming growth factor （

关 键 词：水飞蓟素 脂肪肝 酒精性 自由基 肿瘤坏死因子Α 转化生长 因子β 

分 类 号：R575.5[医药卫生—消化系统]