阴道毛滴虫铁氧还蛋白基因真核表达质粒的构建  

Construction of Eukaryotic Expression Recombinant Plasmid of Trichomonas vaginalis Ferredoxin Gene

作　　者：谢辉[1] 王雅静[1] 帖超男[1] 毕世樑[2] 刘佩娜[1] 

机构地区：[1]四川大学华西基础医学及法医学院寄生虫学教研室 [2]四川大学华西第二医院,成都610041

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第1期72-73,共2页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：作者用螯合树脂(Chelex-100)提取阴道毛滴虫基因组DNA,PCR扩增出阴道毛滴虫铁氧还蛋白(ferredoxin,Fd)基因,克隆入pMD-18T载体,亚克隆至pcDNA3·1(+)真核表达质粒。经PCR及酶切鉴定,Fd基因体外扩增产物为306bp,与已知序列吻合。成功构建了Fd基因的真核表达质粒。Total DNA was extracted from T. vaginalis with Chelex-100 method and used as templates for PCR. The ferredoxin gene was directionally cloned into plasmid pMD-18T vector and subcloned into eukaryotic expression vector pcDNA3. 1 （ ＋ ）. The transformants were screened and identified by PCR and restriction analysis. The size of amplified ferredoxin gene was 306bp and the DNA sequence of cloned gene was same with that published.

关 键 词：阴道毛滴虫 铁氧还蛋白 真核表达 质粒 基因克隆 

分 类 号：R382.211[医药卫生—医学寄生虫学]