实验性变态反应性神经炎的大鼠淋巴细胞钾离子通道与细胞及体液免疫的关系  

Correlations Between Lymphocyte Potassium Channels and Cellular/Humoral Immunity in Experimental Allergic Neuritis Rats

作　　者：李建萍[1] 吕传真[1] 

机构地区：[1]复旦大学附属华山医院神经内科,200040

出　　处：《中国临床神经科学》2006年第2期139-145,共7页Chinese Journal of Clinical Neurosciences

摘　　要：目的:观察淋巴细胞K+通道在实验性变态反应性神经炎(EAN)大鼠中的表达情况及其与细胞和体液免疫的关联。方法:RT-PCR测定淋巴结和脾脏细胞Kv1.3和IKCa1的mRNA表达量,流式细胞分析相应细胞的增殖能力,间接ELISA测定特异性抗体水平,计算相关系数。结果:淋巴细胞Kv1.3和IKCa1的表达水平与细胞增殖能力紧密相关,与EAN临床严重程度无关,在一定程度上反应了EAN的总体细胞免疫水平。结论:淋巴细胞K+通道可以在以细胞免疫为主导的自身免疫性疾病中作为一个潜在的干预靶点。Aim： To observe the expression of lymphocyte voltage-gated K^＋ channel （Kvl.3） and intermediate-conductance calcium-activated K^＋ channel （IKCal） in experimental allergic neuritis （EAN） rats and its correlations with cellular/humoral immunity. Methods： EAN was induced in Lewis rats with bovine peripheral myelin （BPM） and clinical severity was assessed. On day 9, 16, 23 and 30 post immunization, we evaluated the expression of mRNA for Kvl.3 and IKCal in lymphocytes from inguinal lymph nodes and spleens, the dividing cell proportions of two kinds of lymphocytes in different culture conditions, and the levels of anti-BPM antibodies. Bivariate correlations mRNA expression of lymphocyte K^＋ channels was were performed in statistical analysis. Results： The closely correlated with the capacity of lymphocyte proliferation but had little correlation with EAN clinical severity, which was able to reflect the cellular immunity status of EAN on certain extent. Conclusions： Lymphocyte K^＋ channels may be a potential therapeutical target in autoimmune diseases mediated by cellular immunity.

关 键 词：实验性变态反应性神经炎 淋巴细胞 钾离子通道 

分 类 号：R745.4[医药卫生—神经病学与精神病学]