Notch 1信号蛋白在大鼠牙胚发育中的免疫组化表达研究  

The expressiou of Notch 1 as signal during rat teeth development in dentin

作　　者：郭卫拉 赵守亮 臧小霞 

机构地区：[1]解放军第四军医大学口腔医院牙体科,西安710032

出　　处：《现代口腔医学杂志》2006年第2期167-169,共3页Journal of Modern Stomatology

摘　　要：目的通过检测大鼠牙胚发育不同阶段Notch 1信号的表达情况,探讨其在牙齿发育过程中的作用。方法采用免疫组织化学染色技术观测大鼠孕期12、14、16、17、18、19d和出生后1、3、5、7、9d牙胚中Notch 1的表达。结果Notch 1的表达始于钟状中期。在钟状中晚期,Notch 1的表达在牙乳头间充质、成牙本质细胞层呈弱阳性,在内釉细胞层呈阳性。牙体组织形成开始,Notch 1则在成釉细胞的中间层有反复表达,在成牙本质细胞层中有一过性表达。牙齿萌出后,该信号在成牙本质细胞和成釉细胞表达均为阴性。结论Notch 1在牙胚发育过程中与成釉细胞分化有关,它可能在牙齿发育早期基质触发前成釉细胞向成釉细胞分化中起重要作用。Objective To discuss the probable role of Notch 1 as signal in rat tooth development. Methods The expression of Notch 1 as signal was observed by Immunohistochemistry technique in tooth germ at different developmental stages,which were E14,E17,E19 and 1d,3d,Sd,7d postnataUy. Results The expression of Notch 1 was initiated in early bell stage in inner - enamal epithelium pre - odontoblasts and dental papilla mesenchymal cells. Et was repeatedly expressed in ameloblasta after dental tissue was formed. And negtive in both odontoblasta and ameloblasta after tooth eruption. Conclusion Notch 1 is involved in tooth development. It may be related to the formatim of crown hard tissue. And also play an important role in matrix secetion in early development stage and the triggering of transition from pre- ameloblasts to ameloblasts.

关 键 词：NOTCH 1 大鼠 牙胚发育 

分 类 号：R780.2[医药卫生—口腔医学]