原发性胆汁性肝硬化患者CD11c^+和CD123^+树突状细胞与肝功能损伤的关系  

Relations of peripheral blood CD11c^+ and CD123^+ dendritic cells with hepatic injury in patients with primary biliary cirrhosis

作　　者：李永哲[1] 胡朝军 佟大伟 张蜀澜[1] 刘定华 

机构地区：[1]中国医学科学院中国协和医科大学

出　　处：《世界华人消化杂志》2006年第3期341-345,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30471617;国家863计划资助项目;No.2002AA2Z2011~~

摘　　要：目的:检测原发性胆汁性肝硬化(PBC)患者外周血树交状细胞亚群CD11c+和CD123+, 探讨其与肝功能损伤及抗线粒体抗体亚型 2(AMA-M2)抗体产生的关系．方法:流式细胞术检测PBC患者(n=40)外周血树突状细胞亚群CD11c+和CD123+比例, 以40例肝脏疾病患者作为疾病对照组,30例健康体检者作为正常对照组,观察CD11c+和 CD123+树突状细胞与患者的肝功能指标及 AMA-M2抗体产生的关系．结果:PBC患者外周血CD11c+和CD123+比例显著低于正常对照组(0．087 2±0．008 2 vs 0．169 0±0．011 3,P<0．01;0．034 9±0．004 9 vs 0．064 3±0．005 4,P<0．01)．肝功严重损伤的 PBC患者外周血CD11c+及CD123+比例显著低于轻度损伤者(0．071 6±0．007 3 vs 0．124 2 ±0．0094,P<0．01;0．042 6±0．005 9 vs 0．061 7 ±0．006 1,P<0．01)．AMA-M2+患者外周血 CD11c+和CD123+比例显著低于AMA-M2- 患者(0．076 1±0．005 1 vs 0．096 5±0．008 3, P<0．05;0．046 6±0．006 9 vs 0．063 1±0．005 7, P<0．05)．经动态观察发现,同一PBC患者经过治疗后CD11c+和CD123+比例增加,特别是 CD123+明显高于治疗前(0．058 3±0．004 9 vs 0．032 1±0．004 1,P<0．01)．结论:PBC患者外周血树突状细胞亚群 CD11c+和CD123+比例与肝功能损伤和血清抗AMA-M2抗体产生有密切关系．CD11c+和 CD123+的变化可能是导致肝功能损伤和病情发展及血清抗AMA-M2抗体产生的环节之一．AIM： To investigate the levels of CD11c^＋ and CD123^＋ dendritic cells in the peripheral blood and their associations with hepatic injury in patients with primary biliary cirrhosis （PBC）. METHODS： The percentages of CD11c^＋ and CD123^＋ dendritic cells in the peripheral blood from patients with PBC （n = 40）, other hepatic diseases （n = 40） and normal individuals （n = 30） were measured by flow cytometry. RESULTS： The levels of CD11c^＋ and CD123^＋ dendritic cells in patients with PBC were significantly lower than that in normal controls （0.087 2 ± 0.008 2 vs 0.169 0 ± 0.011 3, P 〈0.01; 0.034 9 ± 0.004 9 vs 0.064 3 ± 0.005 4, P 〈0.01）. The ratios of CD11c^＋ and CD123^＋ dendritic cells in patients with severe hepatic injury were markedly lower in comparison with that of patients without severe hepatic injury （0.071 6 ±0.007 3 vs 0.124 2 ± 0.009 4, P 〈0.01; 0.042 6 ± 0.005 9 vs 0.061 7 ± 0.006 1, P 〈0.01）. Furthermore, PBC patients with AMA-M2 positive had statistically lower levels of CD11c^＋ and CD123^＋ in peripheral blood than those with AMA-M2 negative did （0.076 1 ± 0.005 1 vs 0.096 5 ± 0.008 3, P 〈0.05; 0.046 6 ± 0.006 9 vs 0.063 1 ± 0.005 7, P 〈0.05）. Dynamically, we found the levels of CD11c^＋ and CD123^＋ dendritic cells in the same PBC patients were increased after treatment, while CD123^＋ dendritic cells was notably increased （0.058 3 ± 0.004 9 vs 0.032 1 ± 0.004 1,P 〈0.01）. CONCLUSION： The levels of peripheral blood CD11c^＋ and CD123^＋ dendritic cells in patients with PBC are correlated with hepatic injury and serum anti-AMA-M2 antibody production. The dendritic cell subtypes CD11c^＋ and CD123^＋, especially CD123^＋, may play crucial roles in the pathogenesis and progression of hepatic injury.

关 键 词：原发性胆汁性肝硬化 树突状细胞 CD11c^+ CD123^+ 

分 类 号：R575.2[医药卫生—消化系统] R392.4[医药卫生—内科学]