维生素D受体Taq基因多态性与糖尿病视网膜病变的关系  

The relationship between vitamin D receptor gene TaqⅠ polymorphism and diabetic retinopathy

作　　者：孙京华[1] 陈艳艳[1] 刘常明[1] 张熠昭[1] 王慧[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼科,武汉430030

出　　处：《中华眼底病杂志》2006年第2期94-96,共3页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的探讨维生素D受体(VDR)Taq基因多态性与糖尿病视网膜病变(DR)的关系。方法应用片段长度差异等位基因特异性多聚酶链反应(FLDAS-PCR)技术,检测158例DR患者和198名正常对照者的VDRTaq基因多态性。结果VDRTaq基因型在DR患者中的分布为TT型106例,占67.1%,Tt型33例,占20.9%,tt型19例,占12.0%;在正常对照者中的分布为TT型165例,占83.3%,Tt型23例,占11.6%,tt型10例占5.1%。DR患者与正常对照者TT、Tt、tt基因型分布差异有统计学意义(P<0.05)。结论DR与维生素D受体Taq基因多态性有一定的关系。Objective To investigate the polymorphism of the vitamin D receptor gene （VDR）Taq 1 in relation to diabetic retinopathy. Method Fragment length discrepant allele specific PCR（FLDAS- PCR） were used to determine VDR genetypes in 158 patients with diabetic retinopathy and in 198 normal subjects. Results The frequency distribution of VDR genotypes in diabetic retinopathy patients was 106 （67.1%） in TT, 33（20. 9%） in Tt, 19（12.0%） in tt; and in normal persons was 165 （83.3%） in TT, 23 （11.6%） in Tt, 10 （5.1%） in tt. There was a significant difference between diabetic retinopathy patients and normal persons in distribution of VDR gene Taq I genotypes （P〈 0. 05）. Conclusions There is some distribution alterations of VDR gene polymorphism in diabetic retinopathy patients.

关 键 词：糖尿病视网膜/遗传学 受体 骨化三醇 基因调控区 多态性 单核苷酸 聚合酶链反应 

分 类 号：R774.1[医药卫生—眼科]