培养基和培养条件对葡萄试管苗生根的影响  

Effects of different media and culture conditions on rooting in vitro of test-tube plantlets of Vitis vinifera L.

作　　者：李胜[1] 张真[1] 李婷[1] 杨广兴[1] 武季玲[1] 杨德龙[1] 曹孜义[1] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《甘肃农业大学学报》2006年第1期24-29,共6页Journal of Gansu Agricultural University

基　　金：甘肃省自然科学基金资助项目(32S051-A25-063)

摘　　要：以矢荚罗莎等4种葡萄试管苗为材料，研究了蔗糖浓度、琼脂浓度、pH、光强及温度对葡萄试管苗生根的影响．结果表明：蔗糖浓度为15～20g／L对葡萄试管苗的生根最有利；pH值对试管苗平均根系数量、根粗、根长和根冠比影响显著。生根数与平均根长随pH值升高而减小，平均根粗与根冠比随pH值的增大而增大；琼脂浓度显著影响葡萄试管苗生根．随着琼脂浓度的增加，根源基产生时间延迟，平均根长和根冠比降低，而平均根粗和生根数增加；生根数在3种温度条件下无显著差异，平均根粗随温度的升高略有下降，平均根长和根冠比随培养温度的升高而增加，在35℃时降低；平均生根数、根长、根冠比和根粗均随光照强度的增强而增大．Effects of different culture conditions including different concentration of sucrose and agar added in media, different pH values, culture temperature and light intension on rooting in vitro of test-tube plantlets of Vitis vinifera L. were carried out . The results showed that the optimum concentration of sucrose for rooting was 15-0 g/L. pH value had significant effects on root number, root diameter, root length and root-shoot ratio of test-tube plantlets cultured with different media, pH value increased, root number and average root length decreased, root diameter and root-shoot ratio increased. Concentration of agar significantly affected the rooting of test-tube plantlets, the high agar concentration was the later root primordium produced, meanwhile, as concentration of agar increased,average root length and root-shoot ratio decreased, average root diameter and root number increased. Root number showed no significant differences among three temperature treatments, while the temperature increased, average root diameter decreased a little,average root length and root-shoot ratio increased first, but when temperature reached to 35 ℃ ,they decreased. With the increasing of light intension, number, length and diameter of root and root- shoot ratio were all increased.

关 键 词：葡萄 离体生根 培养基 PH值 培养条件 

分 类 号：S663.1[农业科学—果树学]