人肝细胞癌与癌旁肝细胞增殖活性的定量研究  

Quantitative study of proliferative activity of human hepatocellular cancerous cells and cancer-adjacent hepatocytes

作　　者：张新立[1] 史景泉[1] 王晓东[1] 王东[1] 

机构地区：[1]第三军医大学基础部病理学教研室

出　　处：《第三军医大学学报》1996年第1期36-39,共4页Journal of Third Military Medical University

摘　　要：采用增殖细胞核抗原（ＰＣＮＡ）免疫组化标记与图像分析技术，对３０例人体肝细胞癌与癌旁近癌肝细胞的增殖活性进行了定量研究。结果显示：（１）癌与癌旁近癌肝细胞ＰＣＮＡ阳性平均标记率分别为４５．２２％±１７．９０％和１２．７４％±４．８９％（Ｐ＜０．０１），随肝癌分级升高，ＰＣＮＡ标记享有升高的趋势。（２）４０％以上肝癌细胞的ＰＣＮＡ相对含量与癌旁肝细胞ＰＣＮＡ平均含量相似，结合病理形态分析，认为癌细胞ＰＣＮＡ含量增高与低分化癌细胞核增大关系密切。（３）用图像分析技术显示的ＰＣＮＡ标记数密度与常规计数法所得ＰＣＮＡ标记率有较好一致性，且前者更为客观。The proliferative:activity of hepatocellular cancerous cells(HCCs)and cancer-adjacent hepa tocytes were quantitatively studied with immunohistochemical assay of the proliferating cell nuclear antigen(PCNA)and computerized image analysis.The findings were as follows:(1)The average rate of PCNA labelling was 45.22%±17.90%and 12.74%±4.89%in HHCs and cancer-adjacent hepatocytes respectively(P<0.01)and the rate of PCNA labelling tended to increase with the increase of the pathological grading of hepatocellular carcinoma,(2)The relative contnet of PCNA in 40%of HCCs was similar to the average content of PCNA of cancer-adjacent hepatocytes.In combination to the histopathological findings,it is concluded that the increase of PCNA content in HCCs was closely related to the increase of their nuclear size,(3)A coincidence was obtained between the numeral density of PCNA-positive cells with image analysis and the labelling rate with routine counting and the former was considered as a more objective index.

关 键 词：肝肿瘤 增殖细胞核 免疫组化 癌旁肝细胞 

分 类 号：R735.703[医药卫生—肿瘤]