NO_3^-胁迫及恢复对黄瓜幼苗叶片叶绿素荧光参数及ATPase活性的影响  

Effects of NO_3^-stress and recovery on chlorophyll fluorescence parameters and ATPase activities of cucumber seedling leaves.

作　　者：杨凤娟[1] 王秀峰[1] 魏珉[1] 苏秀荣[2] 闫童[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,泰安271018 [2]山东农业大学化学与材料科学学院,泰安271018

出　　处：《应用生态学报》2006年第3期403-407,共5页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金资助项目(30471187).

摘　　要：通过水培试验,探讨了不同NO3-浓度胁迫及恢复对黄瓜幼苗叶片叶绿素含量、叶绿素荧光参数及ATPase活性的影响.结果表明,胁迫7 d后,高浓度NO3-(168 mmol.L-1)可极显著提高叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,极显著提高初始荧光(Fo)、Mg-ATPase和Ca-ATPase活性,而PSⅡ原初光能转化效率(Fv/Fm)、PSⅡ潜在活性(Fv/Fo)和PSⅡ光合电子传递量子效率(ΦPSⅡ),却随NO3-浓度的增加而降低.恢复7 d后,所有处理叶绿素和类胡萝卜素含量均低于对照;初始荧光基本都恢复至对照水平;PSⅡ原初光能转化效率和PSⅡ光合电子传递量子效率在NO3-浓度低于126 mmol.L-1时,基本恢复至对照水平,而高于这一水平时,仍显著低于对照;PSⅡ潜在活性在NO3-浓度为42和126 mmol.L-1的处理基本达对照水平,其它处理仍极显著低于对照;Mg-ATPase和Ca-ATPase活性均出现先降低后升高的变化趋势.With water culture, this paper studied the effects of NO^3 - stress and recovery on the chlorophyll contents, chlorophyll fluorescence parameters, and ATPase activities of cucumber seedling leaves. The results showed that under stress for 7 days, the chlorophyll a, chlorophyll b, total chlorophyll and carotenoid contents, intrinsic fluorescence （Fo）, and Mg-ATPase and Ca-ATPase activities were increased significantly when the NO^3- concentration was high （ 168 mmol·L^- 1 ）, while the intrinsic photochemical efficiency （ Fv/Fm ）, potential activities （ Fv/Fo ） and quantum yield （ФPSⅡ） of PS Ⅱ were decreased with increasing NO^3- concentration. After recovery for 7 days, the chlorophyll and carotenoid contents of all treatments with NO^3 - were lower than CK, while the intrinsic fluorescence, intrinsic photochemical efficiency and quantum yield of PSⅡ were similar to CK when the NO^3 - concentration was ≤84 mmol·L^-1, and the Fv/Fm and ФPSⅡ were significantly lower than CK when the NO^3- concentration was〉84 mmol·L^-1. The potential activity of PSⅡ was similar to CK when the NO^3- concentration was 42 and 126 mmol·L^- 1, but significantly lower than CK in other treatments. The activities of Mg-ATPase and Ca-ATPase were decreased firstly but increased then when the NO^3 - concentration was 〉 84 mmol·L^-1.

关 键 词：NO3^-胁迫 黄瓜 叶绿素 荧光参数 ATPASE 

分 类 号：S642.2[农业科学—蔬菜学]