维甲酸β受体的表达介导肺腺癌细胞系A549凋亡的研究  

Expression of retinoic acid receptor β mediates retinoic acid-induced growth arrest and apoptosis in lung cancer cell line A549

作　　者：李尊岭[1] 于雪艳[2] 胡国强[3] 张世国[4] 贺美兰[2] 徐霞[2] 

机构地区：[1]滨州医学院生物化学与分子生物学教研室,山东滨州256603 [2]山东大学医学院生物化学与分子生物学研究所,山东济南250012 [3]山东大学齐鲁医院胸外科,山东济南250012 [4]济南军区总医院检验科,山东济南250012

出　　处：《山东大学学报（医学版）》2006年第1期5-9,14,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然基金资助课题(39770730)

摘　　要：目的:研究RARβ在肺癌细胞的增殖、分化和诱导细胞凋亡中的作用。方法:应用脂质体将RARβ的表达载体pcDNA3.0-RARβ瞬时转染到RARβ表达下降的肺腺癌细胞A549中,观察形态学变化;应用流式细胞仪检测细胞凋亡率;RT-PCR检测细胞RARβ、p53、cdk4、cyclinD1的表达水平。结果:试验组出现细胞皱缩、粘附性降低、染色体凝集和边集现象,细胞凋亡率为65.8%,DNA断裂成片状。RT-PCR显示凋亡细胞中的RARβ表达明显升高(P<0.01)、p53的表达无明显变化、cdk4和cyclinD1的表达明显降低(P<0.01)。结论:9-顺-维甲酸通过RARβ受体诱导细胞凋亡并且显著降低cdk4、cyclinD1的表达;9-顺-维甲酸不影响细胞中p53的表达;9-顺-维甲酸在p53表达正常的细胞中引起凋亡。Objective： To investigate the role of RARβ in regulating the proliferation,differentiation,and apoptosis in lung cancer ceils. Methods： With lipofectain the expression vector, pcDNA3.0 was instantaneously transfected into the lung adenoeareinoma cell line A549 which was down-regulated expressed by RARβ. With inverted phase microscope the cells morphological changes were observed and the apoptosis was detected. Meanwhile with RT-PCR technique, the expressions of RARe, cdk4, cyclinD1 and p53 gene were observed. Results： The experimental cells showed cell shrinkage,loss of adhesion and nuclear condensation; the coefficient rate of apoptosis was 65.8%; DNA was fragmented. RT-PCR result showed that the expression level of RARβ was highed,the level of p53 was not changed and the level of cdk4 and cy- clinD1 was lowered. Conclusion： 9-cis-RA induces the apoptosis of the lung cancer cells by RARβ; the cooperation of 9-cis-retinoid acid and RARI3 brings down the expression of cdk4 and cyclinD1 significantly; 9- cis-RA has no effect in the expression of p53; 9-cis-RA results in apoptosis of cells that express the normal p53.

关 键 词：维甲酸 受体 维甲酸 肺肿瘤 信号传导 细胞周期蛋白类 

分 类 号：R734.2[医药卫生—肿瘤]