花药培养技术在杂交大麦三系选育中的应用  

SELECTION AND BREEDING OF "THREE LINES"IN HYBRID BARLEY BY ANTHER CULTURE

作　　者：杜永芹[1] 陈如梅[1] 张国荣[1] 黄培忠[1] 马俊虎[1] 李承道[1] 

机构地区：[1]上海市农业科学院作物育种栽培研究所

出　　处：《上海农业学报》1996年第2期10-12,共3页Acta Agriculturae Shanghai

基　　金：上海市启明星项目

摘　　要：以瑞典引进的大麦核质互作型雄性不育三系为试材，与当地大麦品种（系）杂交，并于Ｆ１代进行花药培养。由花药培养再生的植株经加倍后，筛选出４个具开颖、抗大麦黄花叶病（ＢａＹＭＶ）的保持系９３Ｈ２５９２、９３Ｈ２５９３、９３Ｈ２５７３、９３Ｈ２５７４；培育出不育率在９７．２０％～９８．４８％的９３０７５３－１、９３０７５３－２、９３０７５３－３及９３０７５４－１四个不育系和恢复结实率大于９５．００％的９４Ｈ４７７１Ｂ２、９４Ｈ０８２２Ｂ４二个恢复系。Three lines' of karyocytoplasmic interaction type MS from Sweden were crossed with local barley varieties,anthers of F, hybrids were cultured. The following lineswere screened: four MS maintainer lines'93H2592, 93H2573, 93H2593, 93H2574' with open glumes and resistance to BaYMV: four MS lines '930753-1, 930753-2, 930753-3, 930754-1' with sterility rates from 97. 2% to 98. 4%; and two MS restorer lines u 94H4771B2,94H0822B4' with restoration rates more than 95%.

关 键 词：大麦 花药培养 三系配套 选择育种 

分 类 号：S512.303.5[农业科学—作物学]