AP－PCR在肿瘤DNA多态性研究中的应用  

Application of AP-PCR in polymorphic DNA analysis in carcinomas

作　　者：陈燃[1] 杨树青[1] 王世平 温俊娥 毛裕民[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《复旦学报（自然科学版）》1996年第2期222-227,共6页Journal of Fudan University：Natural Science

摘　　要：首次将ＡＰ－ＰＣＲ应用于ＤＮＡ多态性研究．研究ＡＰ－ＰＣＲ的反应程度，找到实现稳定扩增的适宜条件，并发现模板ＤＮＡ浓度的变化在相当范围内不会改变扩增图谱；对肿瘤、瘤周组织以及正常组织进行分析，在１１个引物中发现２个引物的扩增结果是多态的．初步结论：多态现象与癌变相关．P-PCR,a new molecular technique was used to amplify polymorphic DNA of carcinoma in this paper. We systematically studied the experimental protocols and found out an optimum program for carcinoma. Changes of the concentration of templet DNA does not change patterns of products of the AP-PCR within a wide range. 11primers were used,and 2 primers could amplify the polymorphic DNA of carcinoma. It was suggested that the polymorphic products might relevant to carcinogenesis.

关 键 词：肿瘤 AP-PCR DNA 多态性 癌变 瘤周组织 

分 类 号：R730.21[医药卫生—肿瘤]