褪黑素对睡眠剥夺大鼠学习记忆和行为的影响  

Effects of melatonin on learning and memory ability and behavior of rats in sleep deprivation

作　　者：吴琼[1] 刘芳娥[2] 张玉明[1] 刘利兵[2] 李静[3] 贾锋涛[1] 王斌[1] 

机构地区：[1]第四军医大学口腔医学系11队,西安710032 [2]第四军医大学基础部教学实验中心,西安710032 [3]第四军医大学预防医学系放射医学教研室,西安710032

出　　处：《中国行为医学科学》2006年第3期216-218,共3页Chinese Journal of Behavioral Medical Science

摘　　要：目的研究褪黑素对睡眠剥夺(SD)大鼠记忆能力和行为的影响。方法SpragueDawley大鼠24只随机分为3组;实验1组:SD+褪黑素(5mg/kg),实验2组:SD+褪黑素(15mg/kg),对照组:SD+生理盐水。持续给药15d后开始睡眠剥夺,SD48h和72h后用水迷宫测试大鼠学习记忆能力,SD72h后用旷场反应箱测试大鼠的行为。结果SD48h和72h后,与对照组[(35.02±17.84)s,(23.80±18.66)s]相比,实验1组[(18.38±11.49)s,(8.47±6.65)s]与实验2组[(4.93±2.92)s,(5.40±6.04)s]大鼠水迷宫测试的逃避潜伏期均显著缩短(P<0.05,P<0.01)。实验1组,实验2组大鼠在旷场反应箱中运动总距离分别为[(2793.6±893.3)cm,(2016.5±753.9)cm];离开边界总距离分别为[(392.3±257.7)cm,(256.3±296.0)cm],均明显小于对照组[(3755.0±551.1)cm,(802.0±271.4)cm](P<0.05、P<0.01)。结论褪黑素对SD所致的大鼠学习记忆障碍有改善作用,且对SD大鼠中枢兴奋性的提高有抑制作用。Objective To explore the effects of melatonin （MT） on the memory ability and behavior of rats in sleep deprivation. Methods 24 rats were randomly divided into 3 groups. Experimental group 1 ：SD ＋ MT（5mg/kg） ; experimental group 2：SD ＋ MT（15mg/kg） ;control group： SD ＋ normal saline. Rats were given sleep deprivation after 15 days＇ continue medication, learning and memory ability in rats were measured by water maze after 48h and 72h SD, and behavior was measured by ＂open field test＂ after 72h SD. Results After 48h and 72h SD, compared with the control group [ （ 35.02 ± 17.84） s, （ 23.80 ± 18.66 ） s ] , the escape delitescences in water maze test of experimental groupl [ （ 18.38 ± 11.49 ） s, （ 8.47 ± 6.65 ） s ] and experimental group2 [ （4.93 ± 2.92） s, （5.40 ± 6.04） s ] were both decreased（ P 〈 0.05, P 〈 0.01 ）. The Total Distances in the ＂open field＂ of the control group, experimental group l and experimental group2 separately are （2793.6 ±893.3 ）era, （2016.5 ± 753.9 ） cm ; and the Leave Border Distances separately are （ 392.3 ± 257.7 ） cm, （ 256.3 ± 296.0） era,which were both decreased compared to the control group [ （ 3755.0 ± 551.1 ） cm, （ 802.0 ± 271.4 ） cm ]. Conclusion Melatonin can improve the learning and memory dysmnesia induced by sleep deprivation, and the excitability in rats of sleep deprivation can be inhibited.

关 键 词：睡眠剥夺 褪黑素 学习记忆 行为 

分 类 号：R740[医药卫生—神经病学与精神病学]