Pristane诱导BALB/c狼疮鼠模型的实验研究  

Experimental Study of Pristane-induced Murine Lupus Model

作　　者：崔广梅[1] 刘钢[1] 刘渭[1] 阚兵[2] 毛咏秋[2] 魏于全[2] 

机构地区：[1]四川大学华西医院临床免疫科,成都610041 [2]四川大学华西医院人类疾病生物治疗国家重点实验室

出　　处：《四川大学学报（医学版）》2006年第2期309-312,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技厅重点科技项目(03JY029-083-1)资助

摘　　要：目的建立系统性红斑狼疮小鼠模型,探讨环境因素在发病机制中的地位。方法雌性BALB/c小鼠30只,分为两组,造模组一次性腹腔注射Pristane0.5mL,对照组一次性腹腔注射0.5mL生理盐水,注射前及注射后每月定期检测抗核抗体(ANA)、抗双链DNA(dsDNA)抗体,及随机尿蛋白定量,8个月时处死动物,观察肾脏HE染色及直接免疫荧光染色后镜下变化。结果造模组ANA和抗dsDNA抗体分别于造模3个月和4个月时开始出现;8个月时,阳性率分别为87.5%和47.8%;62.5%的小鼠出现≥+(300mg/L)蛋白尿;肾脏组织HE染色及直接免疫荧光染色见肾小球肾炎表现。对照组中各有1只小鼠分别为ANA、抗dsDNA抗体低滴度阳性;随机尿蛋白定量均≤+;肾脏组织未见病理改变。抗dsDNA抗体与蛋白尿之间存在一定相关性(r=0.538,P=0.003)。结论Pristane成功诱导系统性红斑狼疮动物模型。Objective To establish a mouse model of systemic lupus erythematosus（SLE） and to discuss the role of environmental factors in the pathogenesis of SLE. Methods Thirty 10-week-old female BALB/c mice were divided into the model group and control group. The model mice received a single intraperitoneal injection of 0. 5 mL of pristane, while control mice received a single i.p. injection of 0. 5 mL of 0. 9% NS. Autoantibodies and proteinuria were detected before injection and monthly thereafter. 8 months after injection, all mice were bled to death. Kidneys were excised to observe the histopathologic evidence of glomerulonephritis. Results After pristane injection, antinuclear antibody（ANA） and anti-dsDNA antibody appeared in sera as early as 3 months and 4 months respectively. At 8 months, the incidence rates of ANA and anti-dsDNA antibody were 87. 5% and 47.8% respectively. The protein concentration of urine in most mice was ≥1＋ （300 mg/L）. Light microscopy and immunofluorescence of kidney sections indicated glomerulonephritis. In control group, only one serum was ANA low-titer positive and another was anti-dsDNA antibody low-titer positive. The protein concentration of urine was ≤1 ＋. The histopathology and immunofluorescence showed no evidence of glomerulonephritis. Spearman correlation analysis indicated that anti-dsDNA antibody was correlated with proteinuria （r,----0. 538,P=0. 003）. Conclusion The murine lupus model can be successfully established in female BALB/c mouse with a single i.p. injection of 0. 5 mL of pristarie and the specific autoantibody anti-dsDNA for SLE has appeared in sera of BALB/c mice.

关 键 词：系统性红斑狼疮 PRISTANE 动物模型 

分 类 号：R593.241[医药卫生—内科学]