新基因AngRem104对纤粘连蛋白基因上游序列的调控分析  

AngRem104 potentially regulates the transcription of fibronectin through the activation of promoter

作　　者：张宏[1] 梁秀彬[1] 侯平[1] 李卓[1] 王海燕[1] 

机构地区：[1]北京大学第一医院肾内科,北京大学肾脏病研究所北京100034

出　　处：《中国病理生理杂志》2006年第3期417-420,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370651);北京大学"十五""211"工程重点学科建设项目;国家高技术发展计划"863计划"子课题(No.2002BA711A01-18)

摘　　要：目的:探讨新基因AngRem104对纤粘连蛋白(FN)基因启动子转录活性的影响,为揭示AngRem104对FN表达的调控机制提供线索。方法:构建了FN基因上游调控序列的系列缺失体-荧光素酶(luciferase)报告基因,瞬时转染人系膜细胞,检测报告基因的活性变化。结果:FN507-pGL3+正义AngRem104质粒转染组和FN1280-pGL3+正义AngRem104质粒转染组的luciferase相对活性较FN122-pGL3+正义AngRem104质粒转染组luciferase相对活性明显升高(P<0.01)。结论:在FN基因上游调控序列中-122到-507之间存在新基因AngRem104的正反应调控区。AIM： To investigate the mechanism of AngRem104 - mediated regulation of flbronectin gene in human mesangial cells. METHODS： A series of deleted FN promoter sequences was constructed, which fuse to a luciferase reporter gene, and then the potential active regions that respond to AngRem104 in the upstream regulatory sequence of human FN gene was screened by detecting the luciferase activity of promoter - reporter gene. RESULTS： The detection of relative luciferase activity revealed that there was no significant differences when the HMC were transfected with FN122 - reporter gene together with sense AngRem104 construct, but the luciferase activity significantly increased when transfection of FN507 - reporter gene construct together with sense AngRem104 construct. However; no increase in luciferase activity was observed when transfection of FN1280 - reporter gene construct together with sense AngRem104 construct. The potential regulatory region responds to AngRem104 is in the upstream sequence （ - 122 to - 507） of human FN gene. CONCLUSION： Our preliminary study provided the evidence that AngRem104 may mediate the transcription of the FN gene via the activation of its oromoter.

关 键 词：纤连蛋白类 基因 调节 基因 AngRem104 

分 类 号：R363[医药卫生—病理学]