胍基丁胺抑制低氧培养大鼠肺动脉平滑肌细胞增殖  

Agmatine inhibits proliferation of rat PASMCs induced by hypoxia

作　　者：曹学武[1] 高钰琪[1] 

机构地区：[1]第三军医大学高原军事医学系病理生理学与高原生理学教研室

出　　处：《中国病理生理杂志》2006年第3期452-455,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30170405)

摘　　要：目的:观察胍基丁胺对低氧培养大鼠肺动脉平滑肌细胞(PASMCs)增殖的影响。方法:采用组织块贴壁法原代培养大鼠PASMCs,取对数生长期PASMCs分对照组和胍基丁胺组,比色法测定细胞培养液中乳酸脱氢酶(LDH)活力,液闪计数仪测定[3H]-TdR掺入量,流式细胞仪测定细胞周期,图像分析法测定增殖细胞核抗原含量(PCNA)作为细胞增殖的指标。结果:常氧和低氧(2.5%O2)培养时,胍基丁胺组LDH活力与对照组LDH活力均无显著差异(P>0.05);与单纯低氧组相比,50μmol/L胍基丁胺可显著减少低氧培养PASMCs的[3H]-TdR掺入量(P<0.01)。随着胍基丁胺浓度从50μmol/L逐渐增加至500μmol/L,大鼠PASMCs[3H]-TdR的掺入量亦随之减少(P<0.01)。与单纯低氧组相比,胍基丁胺可显著减少低氧培养PASMCs的PCNA的含量(P<0.01),使G0/G1期细胞比例显著增加,G2/M期细胞比例显著减少(P<0.01)。结论:胍基丁胺对PASMCs不产生明显的细胞毒性作用;胍基丁胺可抑制低氧培养大鼠PASMCs的增殖,这种抑制作用呈剂量依赖性。AIM： To determine the effect of agmatine on the proliferation of pulmonary artery smooth muscle cells （PASMCs） induced by hypoxia. METHODS： Primary culture of rat PASMCs was prepared from adult male Wistar rat pulmonary artery by the method of tissue block anchorage. PASMCs were divided into two groups： control group and agmatine- treated group. The activity of LDH in the medium was measured by chromatometry. [^3H] - TdR incorporation was measured by liquid scintillation counting. The cell cycle was measured by flow cytometry, and the PCNA content was measured by image analysis.RESULTS： Agmarine did not exert significant effect on the activity of LDH in the medium, but significantly decreased the [^3H] - TdR incorporation and PCNA content of PASMCs. Agmatine significantly decreased the ceil ratio of G2/M phase and increased the cell ratio of G0/G1 phase. With the increment of the concentration of agmatine, [^3H] - TdR incorporation was significantly decreased. CONCLUSION： Agmatine has no significant cytotoxic effect on PASMCs and agmatine dose- dependently inhibits the proliferation of rat PASMCs induced by hypoxia.

关 键 词：肺动脉 胍丁胺 缺氧 细胞增殖 

分 类 号：R363[医药卫生—病理学]