NOD/SCID小鼠胎盘细胞内细胞因子表达及其与妊娠转归的关系  

Intracellular expression of cytokines in placenta cells from NOD/SCID mice and its relationship with pregnancy outcomes

作　　者：贺芳[1] 曾耀英[1] 林羿[1] 陈艺菁[1] 罗文亭[1] 王通[1] 

机构地区：[1]暨南大学组织移植与免疫研究中心,广东广州510632

出　　处：《中国病理生理杂志》2006年第3期563-566,共4页Chinese Journal of Pathophysiology

基　　金：暨南大学引进优秀人才科研启动基金资助(No.51204067);广东省自然科学基金资助(No.04300213);中国博士后科学基金资助(No.2002032244)

摘　　要：目的:研究Th1和Th2型细胞因子在胎盘淋巴细胞内的表达状况及其与NOD/SCID孕鼠妊娠转归的关系。方法:比较BALB/c和NOD/SCID小鼠孕13.5d胚胎吸收率(RR),采用4色流式细胞术检测胎盘淋巴细胞内Th1型(TNF-α和IL-2)和Th2型(IL-10)细胞因子表达率。结果:虽然NOD/SCID小鼠存在多重免疫缺陷,但BALB/c和NOD/SCID小鼠孕13.5dRR无显著差异;NOD/SCID×NOD/SCID母-胎界面CD8+IL-10+/CD8+细胞百分率显著高于BALB/c×BALB/c妊娠模型(P<0.01),而CD4和CD8阳性细胞内TNF-α和IL-2表达水平均无显著差异。结论:母-胎界面CD8+IL-10+/CD8+细胞百分率自发性升高可能与NOD/SCID小鼠基本正常的生育力有关。AIM：To investigate the relationship between the intracellular expreasion of Th1 and Th2 type cytokines in placental lymphocytes and the pregnancy outcomes in NOD/SCID mice.METHODS：The resorption rate of embryos（RR） was compared between BALB/c and NOD/SCID mice.In addition,the expression rates of Th1 type （TNF-α and IL-2） and Th2 type （IL-10） cytokines were detected intracellularlly in placental lymphocytes by four-color flow cytometry.RESULTS：A lthough multiple-immunodeficiency was confirmed in the NOD/SCID,no significant difference was observed in RR between BALB/c and NOD/SCID mice.At the same time,a dramatically elevated level of CD8^＋ IL-10^＋/CD8^＋ cell percentage was found at the feto-maternal interface in NOD/SCID×NOD/SCID,as compared with BALB/c×BALB/c mice,while no significant difference was observed for TNF-α and IL-2 expression in CD4^＋ and CD8^＋ cell subsets isolated from these mice.CONCLUSION：The spontaneous for TNF-α and IL-2 expression in CD4^＋ and CD8^＋ cell subsets isolated from these mice.CONCLUSION：The spontsneous elevation of CD8^＋ IL-10^＋/CD8^＋ cell percentage at the feto-matemal interface may be related to the roughly normal fertility in NOD/SCID mice.

关 键 词：胎盘 细胞因子类 免疫缺陷 妊娠结局 

分 类 号：R363[医药卫生—病理学]