氯丙米嗪促进局灶性脑缺血损伤大鼠运动功能恢复的特点(英文)  

Effect of chlorimipramine on recovery of motor function of rats with focal cerebral ischemic injury

作　　者：郭俊[1] 刘学东[2] 张晓梅[1] 周永清[1] 刘立[1] 

机构地区：[1]解放军第四军医大学航空航天医学系临床医学教研室神经精神病学组,陕西省西安市710032 [2]解放军第四军医大学西京医院神经内科,陕西省西安市710032

出　　处：《中国临床康复》2006年第12期172-173,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:去甲肾上腺素能药物苯丙胺可提高脑缺血后动物运动功能的恢复。氯丙米嗪抑制5-羟色胺和去甲肾上腺素再摄取,提高脑内去甲肾上腺素和5-羟色胺水平。目的:观察氯丙米嗪对局灶性脑缺血损伤后大鼠运动功能的作用。设计:随机分组设计,对照动物实验。单位:解放军第四军医大学航空临床医学教研室神经精神病学组。材料:实验在第四军医大学航空航天医学系形态学实验室完成。选择SD大鼠24只随机分为假手术组、缺血组、缺血用药组,每组8只。缺血用药组动物于缺血后24h经口灌注2.5g/L氯丙米嗪溶液(10mg/kg),1次/d。假手术组、缺血组动物经口灌注相应容量的蒸馏水。方法:采用插线法制作大脑中动脉缺血/再灌注大鼠模型,造模成功后进行①网屏握持试验:将网屏水平放置,将鼠放于其上,然后缓慢地将其一端抬高,在2s内将此网屏翻转成125°位,并保持于该位,记录大白鼠在网屏上握持的时间。②胶布撕脱试验:将0.5cm2的医用胶布粘于大鼠两前爪腹侧面后,送其入观察箱中,记录其撕去胶布所使用的时间。各组实验动物在术后1,3,7,14,28d为观察时间点。主要观察指标:大鼠局灶性脑缺血后网屏握持时间和胶布撕脱时间。结果:①与假手术组比较,缺血组和缺血用药组大鼠网屏肌力测试时间缩短,差异非常显著眼以术后3d为例,假手术组、缺血组、缺血用药组分别为(54±4),(20±5),(21±4)s,P<0.01演。缺血用药组与缺血组比较网屏握持时间延长,差异显著眼以术后28d为例,缺血组、缺血用药组分别为(51±5),(54±5)s,P<0.05演。②缺血组和缺血用药组大鼠胶布撕脱时间均比假手术组长,差异非常显著眼以术后3d为例,假手术组、缺血组、缺血用药组分别为(47±9),(188±20),(172±22)s,P<0.01演。缺血用药组比缺血组大鼠胶布撕脱时间略短,差异无显著性(P>0.05)。结论:氯丙米嗪对局灶性脑缺BACKGROUND： Amfetamine, one of the levarterenols, can improve the recovery of motor function of animals after cerebral ischemia. Chlorimipramine can inhibit the re-intake of 5-serotonin and levarterenol and improve the level of them in brain. OBJECTIVE： To observe the effect of chlorimipramine on motor function of rats with focal cerebral ischemic injury. DESIGN： Randomized grouping and controlled animal study. SETTING： Neuropsychiatry Team of Aerial Clinical Medical Department of the Fourth Military Medical University of Chinese PLA. MATERIALS： The experiment was completed in the Morphological Laboratory of Aerial Aerospace Medical Department of the Fourth Military Medical University of Chinese PLA. All the 24 SD rats were divided into sham operation group, ischemia group and ischemic medication group with 8 in each group. Rats in ischemic medication group were perfused with 10 mg/kg chlorimipramine solution （2.5 g/L） once a day through mouth 24 hours after ischemia, and rats in sham operation group and ischemia group were perfused with the same volume of distilled water through mouth. METHODS： Middle cerebral artery （MCA） models of ischemia/reperfusion rats were established with inserted lining method. After modeling, the operations were performed as followed： ① holding test of net screen： Net screen was put horizontally, and then rats were put on it. One side of the screen was raised gradually; then it was turned over 125° within 2 s, and maintained at this placement. Time of holding rats on net screen was recorded.② Test of struggling to tear off rubberized cloth： 0.5 cm^2 medical rubberized cloth was adhered to the ventral of anterior claws of rats, and then rats were sent to the observing box to record the time of tearing off rubberized cloth. 1, 3, 7, 14 and 28 days after operation were the observing time points. MAIN OUTCOME MEASURES： Times of holding rats on the net screen and tearing off the rubberized cloth of rats with focal cerebral ischemia. RESULTS： ① Time of mu

关 键 词：脑缺血 运动障碍 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]